Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Laboratory of Chemistry of Natural Molecules, Gembloux Agro-Bio Tech, University of Liege, 5030 Gembloux, Belgium.
Biosensors (Basel). 2021 Sep 24;11(10):354. doi: 10.3390/bios11100354.
For sensitive and fast detection of O157:H7, organic and inorganic hybrid Au/Fe nanoclusters (NCs) were synthesized for the first time using gold nanoparticles (GNPs), bovine serum albumin, ferric chloride, phosphate-buffered saline, and antibodies. The Au/Fe porous spongy NCs with large surface area showed excellent bio-specific capability for O157:H7. GNPs in Au/Fe NCs functioned as signal enhancers, significantly increasing the Raman signal via the metathesis reaction product of Prussian blue and obviously improving the detection sensitivity. We combined the novel Au/Fe NCs with antibody-modified magnetic nanoparticles to create a biosensor capable of sensitive detection of O157:H7, which showed a good linear response (10 to 10 cfu/mL), high detection sensitivity (2 cfu/mL), and good recovery rate (93.60-97.50%) in spiked food samples. These results make the biosensor well-suited for food safety monitoring. This strategy achieves the goal of sensitive and quantitative detection of O157:H7.
为了实现对 O157:H7 的灵敏快速检测，首次利用金纳米粒子（GNPs）、牛血清白蛋白、氯化铁、磷酸盐缓冲盐水和抗体合成了有机-无机杂化 Au/Fe 纳米簇（NCs）。具有大表面积的 Au/Fe 多孔海绵状 NCs 对 O157:H7 表现出优异的生物特异性。Au/Fe NCs 中的 GNPs 作为信号增强剂，通过普鲁士蓝的复分解反应产物显著增加拉曼信号，明显提高了检测灵敏度。我们将新型 Au/Fe NCs 与抗体修饰的磁性纳米粒子结合，创建了一种能够灵敏检测 O157:H7 的生物传感器，该传感器在添加的食品样品中表现出良好的线性响应（10 到 10 cfu/mL）、高检测灵敏度（2 cfu/mL）和良好的回收率（93.60-97.50%）。这些结果使该生物传感器非常适合食品安全监测。该策略实现了对 O157:H7 的灵敏定量检测。
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