基于适配体修饰的 Au@大孔硅基磁光光子微球阵列的高通量、高灵敏、快速表面增强拉曼散射检测大肠杆菌 O157:H7。
High-throughput, highly sensitive and rapid SERS detection of Escherichia coli O157:H7 using aptamer-modified Au@macroporous silica magnetic photonic microsphere array.
机构信息
School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, China.
Medical Imaging Center, the First Affiliated Hospital, Jinan University, Guangdong 510630, China.
出版信息
Food Chem. 2023 Oct 30;424:136433. doi: 10.1016/j.foodchem.2023.136433. Epub 2023 May 23.
The aim of this research was to develop a simple, rapid, sensitive, high-throughput detection method for foodborne Escherichia coli (E. coli) O157:H7 based on the aptamer-modified gold nanoparticles@macroporous magnetic silica photonic microsphere (Au@MMSPM). Such Au@MMSPM array system for E. coli O157:H7 not only integrated sample pretreatment with rapid detection, but also showed highly enhanced effect to develop a highly sensitive SERS assay. The established SERS assay platform gave a wide linear detection range (10-10 CFU/mL) and low limit of detection (2.20 CFU/mL) for E. coli O157:H7. The whole analysis time including sample pretreatment and detection was 110 min. This SERS-based assay platform provided a new high-throughput, highly sensitive and fast detection technology for monitoring E. coli O157:H7 in real samples from the fields of food industry, medicine and environment.
本研究旨在基于适配体修饰的金纳米粒子@介孔磁性硅球（Au@MMSPM），开发一种用于食源性大肠杆菌（E. coli）O157：H7 的简单、快速、灵敏、高通量检测方法。这种用于 E. coli O157：H7 的 Au@MMSPM 阵列系统不仅集成了样品预处理和快速检测，而且对开发高灵敏度 SERS 分析显示出了高度增强的效果。所建立的 SERS 分析平台为 E. coli O157：H7 提供了宽线性检测范围（10-10 CFU/mL）和低检测限（2.20 CFU/mL）。整个分析时间包括样品预处理和检测在内为 110 分钟。该基于 SERS 的分析平台为食品工业、医药和环境领域中实际样品中 E. coli O157：H7 的监测提供了一种高通量、高灵敏度和快速的新型检测技术。
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