Oliveira Pereira Erico A, Labine Lisa M, Kleywegt Sonya, Jobst Karl J, Simpson André J, Simpson Myrna J
Environmental NMR Centre and Department of Physical and Environmental Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, Canada.
Department of Chemistry, University of Toronto, 80 St. George St., Toronto, ON M5S 3H6, Canada.
Metabolites. 2021 Sep 29;11(10):666. doi: 10.3390/metabo11100666.
Bisphenols are used in the production of polycarbonate plastics and epoxy resins. Bisphenol A (BPA) has been widely studied and is believed to act as an endocrine disruptor. Bisphenol F (BPF) and bisphenol S (BPS) have increasingly been employed as replacements for BPA, although previous studies suggested that they yield similar physiological responses to several organisms. is a common model organism for ecotoxicology and was exposed to sub-lethal concentrations of BPA, BPF, and BPS to investigate disruption to metabolic profiles. Targeted metabolite analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to measure polar metabolites extracted from , which are linked to a range of biochemical pathways. Multivariate analyses and individual metabolite changes showed similar non-monotonic concentration responses for all three bisphenols (BPA, BPF, and BPS). Pathway analyses indicated the perturbation of similar and distinct pathways, mostly associated with protein synthesis, amino acid metabolism, and energy metabolism. Overall, we observed responses that can be linked to a chemical class (bisphenols) as well as distinct responses that can be related to each individual bisphenol type (A, F, and S). These findings further demonstrate the need for using metabolomic analyses in exposure assessment, especially for chemicals within the same class which may disrupt the biochemistry uniquely at the molecular-level.
双酚用于聚碳酸酯塑料和环氧树脂的生产。双酚A（BPA）已得到广泛研究，被认为是一种内分泌干扰物。双酚F（BPF）和双酚S（BPS）越来越多地被用作BPA的替代品，尽管先前的研究表明它们对几种生物产生相似的生理反应。斑马鱼是生态毒理学中常用的模式生物，将其暴露于亚致死浓度的BPA、BPF和BPS中，以研究对代谢谱的干扰。通过液相色谱-串联质谱法（LC-MS/MS）进行靶向代谢物分析，以测量从斑马鱼中提取的极性代谢物，这些代谢物与一系列生化途径相关。多变量分析和单个代谢物变化表明，所有三种双酚（BPA、BPF和BPS）都有相似的非单调浓度响应。通路分析表明相似和不同通路受到扰动，主要与蛋白质合成、氨基酸代谢和能量代谢有关。总体而言，我们观察到的反应既可以与一类化学物质（双酚）相关，也可以与每种双酚类型（A、F和S）相关的独特反应有关。这些发现进一步证明了在暴露评估中使用代谢组学分析的必要性，特别是对于可能在分子水平上独特地破坏生物化学的同一类化学物质。
