División de Estudios de Posgrado e Investigación, TecNM/Instituto Tecnológico de Conkal, Av. Tecnológico S/N, Conkal 97345, Yucatán, Mexico.
Unidad Multidisciplinaria de Docencia e Investigación, UMDI-SISAL, Facultad de Ciencias, Universidad Nacional Autónoma de México, Sierra Papacal-Chuburna Km 5, Mérida 97302, Yucatán, Mexico.
Mar Drugs. 2021 Sep 28;19(10):547. doi: 10.3390/md19100547.
Marine gastropods of the genus , comprising more than 800 species, have the characteristic of injecting worms and other prey with venom. These conopeptide toxins, highly diverse in structure and action, are highly potent and specific for their molecular targets (ion channels, receptors, and transporters of the prey's nervous system), and thus are important research tools and source for drug discovery. Next-generation sequencing technologies are speeding up the discovery of novel conopeptides in many of these species, but only limited information is available for , which inhabits sandy mud. To search for new precursor conopeptides, we analyzed the transcriptome of the venous ducts of and identified 55 putative conotoxins. Seven were selected for further study and confirmed by Sanger sequencing to belong to the M-superfamily (Sr3.M01 and Sr3.M02), A-superfamily (Sr1.A01 and Sr1.A02), O-superfamily (Sr15.O01), and Con-ikot-ikot (Sr21.CII01 and Sr22.CII02). Six of these have never been reported. To our knowledge, this report is the first to use high-throughput RNA sequencing for the study of the diversity of conotoxins.
海洋腹足纲动物属，包括 800 多种物种，具有用毒液注射蠕虫和其他猎物的特征。这些结构和作用高度多样化的 conopeptide 毒素对猎物神经系统的分子靶标（离子通道、受体和转运蛋白）具有高度的效力和特异性，因此是重要的研究工具和药物发现的来源。下一代测序技术正在加速许多这些物种中新的 conopeptides 的发现，但对于栖息在沙泥中的，只有有限的信息可用。为了寻找新的前体 conopeptides，我们分析了 的静脉导管的转录组，鉴定出 55 种推定的 conotoxin。选择了 7 种进行进一步研究，并通过 Sanger 测序确认它们属于 M 超家族（Sr3.M01 和 Sr3.M02）、A 超家族（Sr1.A01 和 Sr1.A02）、O 超家族（Sr15.O01）和 Con-ikot-ikot（Sr21.CII01 和 Sr22.CII02）。其中 6 种从未有过报道。据我们所知，本报告首次使用高通量 RNA 测序研究 的 conotoxin 多样性。
