venomomics 揭示了肉食性圆锥蜗牛的毒液复杂性。
Venomics Reveals Venom Complexity of the Piscivorous Cone Snail, .
机构信息
Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Queensland 4068, Australia.
Institut des Biomolecules Max Mousseron, UMR 5247, Université Montpellier-CNRS, 34093 Montpellier, France.
出版信息
Mar Drugs. 2019 Jan 21;17(1):71. doi: 10.3390/md17010071.
The piscivorous cone snail has evolved a net-hunting strategy, akin to the deadly , and is considered the second most dangerous cone snail to humans. Here, we present the first venomics study of venom using integrated transcriptomic and proteomic approaches. Parallel transcriptomic analysis of two specimens revealed striking differences in conopeptide expression levels (2.5-fold) between individuals, identifying 522 and 328 conotoxin precursors from 18 known gene superfamilies. Despite broad overlap at the superfamily level, only 86 precursors (11%) were common to both specimens. Conantokins (NMDA antagonists) from the superfamily B1 dominated the transcriptome and proteome of venom, along with superfamilies B2, A, O1, O3, con-ikot-ikot and conopressins, plus novel putative conotoxins precursors T1.3, T6.2, T6.3, T6.4 and T8.1. Thus, venom comprised both paralytic (putative ion channel modulating α-, ω-, μ-, δ-) and non-paralytic (conantokins, con-ikot-ikots, conopressins) conotoxins. This venomic study confirms the potential for non-paralytic conotoxins to contribute to the net-hunting strategy of
肉食性的芋螺进化出了一种结网捕猎策略，类似于致命的芋螺，被认为是对人类第二致命的芋螺。在这里，我们使用整合的转录组学和蛋白质组学方法首次对 毒液进行了毒液组学研究。对两个 标本的平行转录组分析显示，个体之间的 conopeptide 表达水平存在显著差异（2.5 倍），从 18 个已知基因超家族中鉴定出 522 个和 328 个 conotoxin 前体。尽管在超家族水平上有广泛的重叠，但只有 86 个前体（11%）是两个标本共有的。来自 B1 超家族的 conantokins（NMDA 拮抗剂）主导了 毒液的转录组和蛋白质组，以及 B2、A、O1、O3、con-ikot-ikot 和 conopressins 超家族，加上新的潜在 conotoxin 前体 T1.3、T6.2、T6.3、T6.4 和 T8.1。因此， 毒液包含了麻痹性（假定的离子通道调节 α-、ω-、μ-、δ-）和非麻痹性（conantokins、con-ikot-ikots、conopressins） conotoxins。这项毒液组学研究证实了非麻痹性 conotoxins 可能有助于 的结网捕猎策略。
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