卵泡刺激素受体、抑制素 α、抑制素 βA 和催乳素基因的多态性及其与中国荷斯坦公牛精子质量的关系。

Polymorphisms in follicle stimulation hormone receptor, inhibin alpha, inhibin bata A, and prolactin genes, and their association with sperm quality in Chinese Holstein bulls.

机构信息

China Education Ministry's Key Laboratory in Agricultural Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.

出版信息

Anim Reprod Sci. 2011 Jul;126(3-4):151-6. doi: 10.1016/j.anireprosci.2011.04.023. Epub 2011 Jun 1.

DOI:10.1016/j.anireprosci.2011.04.023

PMID:21684095

Abstract

Candidate genes follicle stimulation hormone receptor (FSHR), inhibin alpha (INHA), inhibin bata A (INHBA) and prolactin (PRL) were investigated for their association with sperm quality traits of semen volume per ejaculate (VOL), sperm concentration (SCON), motility (MOT), sperm motility in frozen semen (FMOT), acrosome integrity rate (AIR) and abnormal sperm rate (ASR) in a total of 123 normal mature Holstein bulls. Three reported single nucleotide polymorphisms (SNPs) of FSHR A-234500T (rs43676359), IHNA A192G (rs41257116), and IHNBA C7639T (rs43408735), and 3 novel SNPs (G7550A, C7661T, and T8370C) in exons 4 and 5 of bovine PRL gene (NC 007324) were analyzed. Analysis of variance revealed that FSHR A-234500T and INHBA C7639T polymorphisms significantly associated with VOL (P<0.05) and SCON (P<0.05), and the polymorphism of INHBA C7639T also had significant effects on MOT (P<0.05). Furthermore, the INHA A192G polymorphism significantly associated with AIR (P<0.05). No significant differences were found between the polymorphisms of PRL gene and sperm quality traits.

摘要

对候选基因卵泡刺激素受体（FSHR）、抑制素 alpha（INHA）、抑制素 bata A（INHBA）和催乳素（PRL）进行了研究，以探讨它们与精液量（VOL）、精子浓度（SCON）、活力（MOT）、冷冻精液中精子活力（FMOT）、顶体完整性率（AIR）和异常精子率（ASR）等精子质量特征的相关性，共涉及 123 头正常成熟荷斯坦公牛。分析了 FSHR 的三个已报道的单核苷酸多态性（SNP）A-234500T（rs43676359）、INHA A192G（rs41257116）和 IHNBA C7639T（rs43408735），以及牛 PRL 基因（NC 007324）外显子 4 和 5 中的 3 个新 SNP（G7550A、C7661T 和 T8370C）。方差分析显示，FSHR A-234500T 和 INHBA C7639T 多态性与 VOL（P<0.05）和 SCON（P<0.05）显著相关，INHBA C7639T 多态性对 MOT 也有显著影响（P<0.05）。此外，INHA A192G 多态性与 AIR 显著相关（P<0.05）。PRL 基因多态性与精子质量特征之间无显著差异。