miR-152通过靶向KLF6调控牛成肌细胞增殖。
miR-152 Regulates Bovine Myoblast Proliferation by Targeting KLF6.
作者信息
Song Chengchuang, Fang Xue, Yang Zhaoxin, Wang Qi, Meng Fantong, Chen Yaqi, Chen Junhao, Zhao Bei, Wang Yanhong, Fang Xingtang, Gu Lihong, Zhang Chunlei
机构信息
Institute of Cellular and Molecular Biology, School of Life Science, Jiangsu Normal University, Xuzhou 221116, China.
Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
出版信息
Animals (Basel). 2021 Oct 19;11(10):3001. doi: 10.3390/ani11103001.
Though miRNAs have been reported to regulate bovine myoblast proliferation, but many miRNAs still need to be further explored. Specifically, miR-152 is a highly expressed miRNA in cattle skeletal muscle tissues, but its function in skeletal muscle development is unknown. Herein, we aimed to investigate the role of miR-152 in regulating bovine myoblast proliferation. Functionally, RT-qPCR, Western blotting, EdU assay, and flow cytometry detection results showed that miR-152 inhibited bovine myoblast proliferation. Mechanistically, we demonstrated transcription factor KLF6 was a target gene of miR-152 by means of bioinformatics software prediction and dual-luciferase report analysis, which had been demonstrated to be favorable for myoblast proliferation. Collectively, our research suggested that miR-152 inhibits bovine myoblast proliferation via targeting KLF6.
尽管已有报道称miRNA可调节牛成肌细胞增殖，但仍有许多miRNA有待进一步探索。具体而言，miR-152是牛骨骼肌组织中高表达的miRNA，但其在骨骼肌发育中的功能尚不清楚。在此，我们旨在研究miR-152在调节牛成肌细胞增殖中的作用。在功能上，RT-qPCR、蛋白质免疫印迹、EdU检测和流式细胞术检测结果表明，miR-152抑制牛成肌细胞增殖。在机制上，我们通过生物信息学软件预测和双荧光素酶报告分析证明转录因子KLF6是miR-152的靶基因，该基因已被证明有利于成肌细胞增殖。总的来说，我们的研究表明miR-152通过靶向KLF6抑制牛成肌细胞增殖。
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