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miR-183/96/182簇通过靶向作用调控牛成肌细胞的发育 。

miR-183/96/182 Cluster Regulates the Development of Bovine Myoblasts through Targeting .

作者信息

Ru Wenxiu, Liu Kunpeng, Yang Jiameng, Liu Jianyong, Qi Xinglei, Huang Bizhi, Chen Hong

机构信息

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China.

Yunnan Academy of Grassland and Animal Science, Kunming 650212, China.

出版信息

Animals (Basel). 2022 Oct 17;12(20):2799. doi: 10.3390/ani12202799.

DOI:10.3390/ani12202799
PMID:36290185
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9597811/
Abstract

Muscle development is an important factor affecting meat yield and quality and is coordinated by a variety of the myogenic genes and signaling pathways. Recent studies reported that miRNA, a class of highly conserved small noncoding RNA, is actively involved in regulating muscle development, but many miRNAs still need to be further explored. Here, we identified that the miR-183/96/182 cluster exhibited higher expression in bovine embryonic muscle; meanwhile, it widely existed in other organizations. Functionally, the results of the RT-qPCR, EdU, CCK8 and immunofluorescence assays demonstrated that the miR-183/96/182 cluster promoted proliferation and differentiation of bovine myoblast. Next, we found that the miR-183/96/182 cluster targeted and restrained its expression. Meanwhile, the expression of had a negative correlation with the expression of the miR-183/96/182 cluster during myoblast differentiation. In a word, our findings indicated that the miR-183/96/182 cluster serves as a positive regulator in the proliferation and differentiation of bovine myoblasts through suppressing the expression of .

摘要

肌肉发育是影响产肉量和肉质的重要因素，并且由多种成肌基因和信号通路协调。最近的研究报道，miRNA作为一类高度保守的小非编码RNA，积极参与调节肌肉发育，但许多miRNA仍有待进一步探索。在此，我们发现miR-183/96/182簇在牛胚胎肌肉中表达较高；同时，它广泛存在于其他组织中。在功能上，RT-qPCR、EdU、CCK8和免疫荧光分析结果表明，miR-183/96/182簇促进了牛成肌细胞的增殖和分化。接下来，我们发现miR-183/96/182簇靶向并抑制其表达。同时，在成肌细胞分化过程中， 的表达与miR-183/96/182簇的表达呈负相关。总之，我们的研究结果表明，miR-183/96/182簇通过抑制 的表达，在牛成肌细胞的增殖和分化中起正调节作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e209/9597811/f44f7b807cd9/animals-12-02799-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e209/9597811/bdebc3df6cf3/animals-12-02799-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e209/9597811/415fda447642/animals-12-02799-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e209/9597811/64b651e2c000/animals-12-02799-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e209/9597811/f44f7b807cd9/animals-12-02799-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e209/9597811/bdebc3df6cf3/animals-12-02799-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e209/9597811/415fda447642/animals-12-02799-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e209/9597811/64b651e2c000/animals-12-02799-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e209/9597811/f44f7b807cd9/animals-12-02799-g004.jpg

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