Hsu Li-Jie, Liu Chao-Lin, Kuo Ming-Ling, Shen Chia-Ning, Shen Chia-Rui
Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan.
PhD Program in Biotechnology Industry, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan.
Biomedicines. 2021 Sep 26;9(10):1323. doi: 10.3390/biomedicines9101323.
Cell therapy is usually defined as the treatment or prevention of human disease by supplementation with cells that have been selected, manipulated, and pharmacologically treated or altered outside the body (ex vivo). Induced pluripotent stem cells (iPSCs), with their unique characteristics of indefinite expansion in cultures and genetic modifications, represent an ideal cell source for differentiation into specialized cell types. Cell therapy has recently become one of the most promising therapeutic approaches for cancers, and different immune cell types are selected as therapeutic platforms. Natural killer (NK) cells are shown to be effective tumor cell killers and do not cause graft-vs-host disease (GVHD), making them excellent candidates for, and facilitating the development of, "off-the-shelf" cell therapies. In this review, we summarize the progress in the past decade in the advent of iPSC technology and review recent developments in gene-modified iPSC-NK cells as readily available "off-the-shelf" cellular therapies.
细胞疗法通常被定义为通过补充在体外（离体）经过选择、操作、药物处理或改变的细胞来治疗或预防人类疾病。诱导多能干细胞（iPSC）具有在培养中无限扩增和基因修饰的独特特性，是分化为特定细胞类型的理想细胞来源。细胞疗法最近已成为治疗癌症最有前景的治疗方法之一，不同类型的免疫细胞被选作治疗平台。自然杀伤（NK）细胞被证明是有效的肿瘤细胞杀手，且不会引起移植物抗宿主病（GVHD），这使其成为“现成可用”细胞疗法的优秀候选者，并推动了此类疗法的发展。在本综述中，我们总结了过去十年诱导多能干细胞技术出现以来的进展，并回顾了基因修饰的诱导多能干细胞来源的自然杀伤细胞作为现成可用的“现货”细胞疗法的最新进展。
