Moerke Caroline, Kloss Marlen, Wulf Katharina, Illner Sabine, Kischkel Sabine, Sombetzki Martina, Grabow Niels, Reisinger Emil, Öner Alper, Ince Hüseyin
Department of Cardiology, University Medical Center Rostock, 18057 Rostock, Germany.
Division of Tropical Medicine and Infectious Diseases, University Medical Center Rostock, 18057 Rostock, Germany.
Biomedicines. 2021 Oct 13;9(10):1464. doi: 10.3390/biomedicines9101464.
Medical device-related infections are becoming a steadily increasing challenge for the health care system regarding the difficulties in the clinical treatment. In particular, cardiovascular implant infections, catheter-related infections, as well as infective endocarditis are associated with high morbidity and mortality risks for the patients. Antimicrobial materials may help to prevent medical device-associated infections and supplement the currently available therapies. In this study, we present an easy-to-handle and simplified in vivo model to test antimicrobial materials in the bloodstream of mice. The model system is composed of the implantation of a bacteria-laden micro-stent scaffold into the murine tail vein. Our model enables the simulation of catheter-related infections as well as the development of infective endocarditis specific pathologies in combination with material testing. Furthermore, this in vivo model can cover two phases of the biofilm formation, including both the local tissue response to the bacterial biofilm and the systemic inflammatory response against circulating bacteria in the bloodstream that detached from a mature biofilm.
对于医疗保健系统而言，与医疗设备相关的感染正成为一个日益严峻的挑战，因为临床治疗存在困难。特别是，心血管植入物感染、导管相关感染以及感染性心内膜炎会给患者带来高发病率和高死亡率风险。抗菌材料可能有助于预防与医疗设备相关的感染，并补充目前可用的治疗方法。在本研究中，我们提出了一种易于操作且简化的体内模型，用于在小鼠血液中测试抗菌材料。该模型系统由将载有细菌的微支架植入小鼠尾静脉组成。我们的模型能够模拟导管相关感染以及结合材料测试的感染性心内膜炎特定病理发展。此外，这种体内模型可以涵盖生物膜形成的两个阶段，包括局部组织对细菌生物膜的反应以及针对从成熟生物膜脱落并进入血液循环的细菌的全身炎症反应。
