冻融后弗朗什-蒙塔涅种公马精子运动性的一个新的 QTL 和候选基因相关。
A Novel QTL and a Candidate Gene Are Associated with the Progressive Motility of Franches-Montagnes Stallion Spermatozoa after Thaw.
机构信息
Animal GenoPhenomics, Agroscope, Route de la Tioleyre 4, 1725 Posieux, Switzerland.
Equine Department, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057 Zurich, Switzerland.
出版信息
Genes (Basel). 2021 Sep 25;12(10):1501. doi: 10.3390/genes12101501.
The use of frozen-thawed semen is an important reproduction tool to preserve the biodiversity of small, native horse breeds such as the Franches-Montagnes (FM). However, not all stallions produce cryotolerant semen with a progressive motility after thaw ≥ 35%. To improve our understanding of the genetic background of male fertility traits in both fresh and frozen-thawed semen, we performed genome-wide association studies (GWAS) on gel-free volume, sperm cell concentration, total sperm count, and progressive motility in fresh and frozen-thawed semen from 109 FM stallions using 335,494 genome-wide single nucleotide polymorphisms (SNPs). We identified one significant ( < 1.69 × 10) quantitative trait locus (QTL) on ECA6 within the gene for progressive motility after thaw, which was previously associated with progressive motility in boars. Homozygous stallions showed a substantial drop in progressive motility after thaw. This QTL could be used to identify cryointolerant stallions, avoiding the costly cryopreservation process. Further studies are needed to confirm whether this QTL is also present in other horse breeds.
使用冻融精液是保存小型本地马品种（如弗朗什-蒙塔涅马）生物多样性的重要繁殖工具。然而，并非所有种马在解冻后都能产生具有≥35%活力的可耐受冷冻的精液。为了更好地了解新鲜和冻融精液中精子活力相关遗传背景，我们使用 335,494 个全基因组单核苷酸多态性（SNP）对 109 匹弗朗什-蒙塔涅马的冻融前精子活力、精液游离量、精子浓度、总精子数进行了无胶体积全基因组关联研究（GWAS）。我们在解冻后活力的基因内鉴定到一个与野猪活力相关的显著（<1.69×10）数量性状位点（QTL），该 QTL 也与解冻后活力相关。纯合种马的解冻后活力显著下降。该 QTL 可用于鉴定对冷冻敏感的种马，避免昂贵的冷冻保存过程。需要进一步研究以确认该 QTL 是否也存在于其他马品种中。
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