Plant Breeding Program, Faculty of Agriculture at Kamphaeng Saen, Kesetsart University, Nakhon Pathom 73140, Thailand.
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Luang, Pathum Thani 12120, Thailand.
Genes (Basel). 2021 Oct 9;12(10):1587. doi: 10.3390/genes12101587.
Rice is one of the most important food crops in the world and is of vital importance to many countries. Various diseases caused by fungi, bacteria and viruses constantly threaten rice plants and cause yield losses. Bacterial leaf streak disease (BLS) caused by pv. () is one of the most devastating rice diseases. However, most modern rice varieties are susceptible to BLS. In this study, we applied the QTL-seq approach using an F population derived from the cross between IR62266 and Homcholasit (HSC) to rapidly identify the quantitative trait loci (QTL) that confers resistance to BLS caused by a Thai isolate, SP7-5. The results showed that a single genomic region at the beginning of chromosome 5 was highly associated with resistance to BLS. The gene was considered a potential candidate gene in this region since most associated single nucleotide polymorphisms (SNPs) were within this gene. A Kompetitive Allele-Specific PCR (KASP) marker was developed based on two consecutive functional SNPs in and validated in six F populations inoculated with another Thai isolate, 2NY2-2. The phenotypic variance explained by this marker (PVE) ranged from 59.04% to 70.84% in the six populations. These findings indicate that is a viable candidate gene for BLS resistance and may help in breeding programs for BLS resistance.
水稻是世界上最重要的粮食作物之一，对许多国家都至关重要。真菌、细菌和病毒引起的各种疾病不断威胁着水稻植株，导致产量损失。由 pv. ()引起的细菌性条斑病 (BLS)是最具破坏性的水稻病害之一。然而，大多数现代水稻品种容易感染 BLS。在这项研究中，我们应用了来自 IR62266 和 Homcholasit (HSC) 杂交的 F 群体的 QTL-seq 方法，快速鉴定出对泰国 分离物 SP7-5 引起的 BLS 具有抗性的数量性状位点 (QTL)。结果表明，染色体 5 起始处的单个基因组区域与 BLS 抗性高度相关。该基因被认为是该区域的一个潜在候选基因，因为大多数相关的单核苷酸多态性 (SNP)都在该基因内。基于 中的两个连续功能 SNP，开发了一种 Kompetitive Allele-Specific PCR (KASP) 标记，并在接种另一个泰国 分离物 2NY2-2 的六个 F 群体中进行了验证。该标记解释的表型方差 (PVE) 在六个群体中范围为 59.04%至 70.84%。这些发现表明 是 BLS 抗性的一个可行候选基因，可能有助于 BLS 抗性的育种计划。
