State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Genes (Basel). 2021 Oct 9;12(10):1591. doi: 10.3390/genes12101591.
(), the larval form of , parasitize mainly the liver, omentum and mesentery of rabbits and cause huge economic losses in the rabbit breeding industry. MicroRNA (miRNA), a short non-coding RNA, is widely and stably distributed in the plasma and serum. Numerous data demonstrates that, after parasitic infection, miRNAs become the key regulatory factor for controlling host biological processes. However, the roles of serum miRNAs in -infected rabbits have not been elucidated. In this study, we compared miRNA expression profiles between the -infected and healthy rabbit serum using RNA-seq. A total of 192 miRNAs were differentially expressed (fold change ≥ 2 and < 0.05), including 79 up- and 113 downregulated miRNAs. These data were verified by qRT-PCR (real time quantitative polymerase chain reaction) analysis. Additionally, GO analysis showed that the target genes of these dysregulated miRNAs were most enriched in cellular, single-organism and metabolic processes. KEGG pathway analysis showed that these miRNAs target genes were involved in PI3K-Akt, viral carcinogenesis and B cell receptor signaling pathways. Interestingly, after aligning clean reads to the genome, four (miR-124-3p_3, miR-124-3p_4, miR-124a and novel-miR1) -derived miRNAs were found. Of these, novel-miR1was upregulated in different periods after infection, which was verified qRT-PCR, and pre- novel-miR-1 was amplified from the cysticerci by RT-PCR, implying novel-miR-1 was derived from and has great potential for the diagnosis of Cysticercosis pisiformis infection. This is the first investigation of miRNA expression profile and function in the serum of rabbits infected by , providing fundamental data for developing diagnostic targets for Cysticercosis pisiformis.
（ ）， 是 的幼虫期，主要寄生于兔的肝脏、大网膜和肠系膜，给养兔业造成了巨大的经济损失。微小 RNA（miRNA）是一种短的非编码 RNA，广泛且稳定地分布于血浆和血清中。大量数据表明，寄生虫感染后，miRNA 成为宿主生物过程调控的关键调节因子。然而，血清 miRNA 在 感染兔中的作用尚未阐明。在本研究中，我们使用 RNA-seq 比较了 感染和健康兔血清中的 miRNA 表达谱。共有 192 个 miRNA 差异表达（fold change≥2 且 < 0.05），包括 79 个上调和 113 个下调 miRNA。这些数据通过 qRT-PCR（实时定量聚合酶链反应）分析得到了验证。此外，GO 分析表明，这些失调 miRNA 的靶基因主要富集在细胞、单细胞生物和代谢过程中。KEGG 通路分析表明，这些 miRNA 靶基因参与了 PI3K-Akt、病毒致癌和 B 细胞受体信号通路。有趣的是，将清洁读取序列与 基因组比对后，发现了 4 个（miR-124-3p_3、miR-124-3p_4、miR-124a 和 novel-miR1） 衍生的 miRNA。其中， novel-miR1 在 感染后不同时期上调，通过 qRT-PCR 得到验证，并且通过 RT-PCR 从囊尾蚴中扩增出 pre- novel-miR-1，这表明 novel-miR1 来源于 ，并且对囊尾蚴病感染的诊断具有很大的潜力。这是首次对 感染兔血清中的 miRNA 表达谱和功能进行研究，为开发囊尾蚴病诊断靶标提供了基础数据。
