Yang Deying, Chen Lin, Xie Yue, Wu Xuhang, Nong Xiang, Peng Xi, Lai Weimin, Gu Xiaobin, Wang Shuxian, Peng Xuerong, Yang Guangyou
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Parasite. 2013;20:53. doi: 10.1051/parasite/2013053. Epub 2013 Dec 12.
The larval stage of Taenia pisiformis, also known as Cysticercus pisiformis, is the causative agent of cysticercosis and the cause of severe health problems in rabbits that negatively impacts on husbandry production. To date, there is no fast detection method to identify early infections in rabbits. In the present study, a new dot-ELISA-based on an endogenous antigen fatty acid-binding protein (FABP) was developed for the detection of cysticercosis, and its potential was then evaluated using test serum samples. Immunolocalisation showed that T. pisiformis FABP (TpFABP) localised to the parenchyma of the bladder wall of the cysticercus and perinuclear cytoplasm of parenchyma of the adult parasite. After cloning and expression, recombinant TpFABP (rTpFABP) protein was used for serodiagnosis of T. pisiformis infection in rabbits by dot-ELISA. The antibody was detected 14 days post-infection in rabbits experimentally infected with T. pisiformis. Based on the necropsy results, the sensitivity and specificity of 169 serum samples tested by rTpFABP dot-ELISA were found to be 98.2% (54/55) and 92.1% (105/114), respectively. These data suggest that the dot-ELISA developed in this study has potential for detection of T. pisiformis infection in rabbits.
豆状带绦虫的幼虫阶段，也被称为豆状囊尾蚴，是囊尾蚴病的病原体，也是导致家兔严重健康问题的原因，对畜牧业生产产生负面影响。迄今为止，尚无快速检测方法来识别家兔的早期感染情况。在本研究中，开发了一种基于内源性抗原脂肪酸结合蛋白（FABP）的新型斑点酶联免疫吸附测定（dot-ELISA）用于检测囊尾蚴病，然后使用测试血清样本对其潜力进行了评估。免疫定位显示，豆状带绦虫FABP（TpFABP）定位于囊尾蚴囊壁实质和成虫寄生虫实质的核周细胞质中。克隆和表达后，重组TpFABP（rTpFABP）蛋白用于通过斑点酶联免疫吸附测定对家兔豆状带绦虫感染进行血清学诊断。在实验感染豆状带绦虫的家兔中，感染后14天检测到抗体。根据尸检结果，经rTpFABP斑点酶联免疫吸附测定检测的169份血清样本的敏感性和特异性分别为98.2%（54/55）和92.1%（105/114）。这些数据表明，本研究中开发的斑点酶联免疫吸附测定在检测家兔豆状带绦虫感染方面具有潜力。
