Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche-Università di Palermo, Via Archirafi 18, 90128 Palermo, Italy.
Istituto per la Ricerca e l'Innovazione Biomedica-Consiglio Nazionale delle Ricerche, Via Ugo La Malfa 153, 90146 Palermo, Italy.
Int J Mol Sci. 2021 Oct 15;22(20):11141. doi: 10.3390/ijms222011141.
Cytochromes P450 (CYP) are enzymes responsible for the biotransformation of most endogenous and exogenous agents. The expression of each CYP is influenced by a unique combination of mechanisms and factors including genetic polymorphisms, induction by xenobiotics, and regulation by cytokines and hormones. In recent years, , one of the closest living relatives of vertebrates, has become a model in various fields of biology, in particular for studying inflammatory response. Using an in vivo LPS exposure strategy, next-generation sequencing (NGS) and qRT-PCR combined with bioinformatics and in silico analyses, compared whole pharynx transcripts from naïve and LPS-exposed , and we provide the first view of cytochrome genes expression and miRNA regulation in the inflammatory response induced by LPS in a hematopoietic organ. In , cytochromes belonging to 2B,2C, 2J, 2U, 4B and 4F subfamilies were deregulated and miRNA network interactions suggest that different conserved and species-specific miRNAs are involved in post-transcriptional regulation of cytochrome genes and that there could be an interplay between specific miRNAs regulating both inflammation and cytochrome molecules in the inflammatory response in .
细胞色素 P450（CYP）是负责大多数内源性和外源性物质生物转化的酶。每种 CYP 的表达受独特的机制和因素组合的影响，包括遗传多态性、外源性物质诱导以及细胞因子和激素的调节。近年来，七鳃鳗作为脊椎动物最亲近的现存亲属之一，已成为生物学各个领域的模型，特别是在研究炎症反应方面。本研究采用体内 LPS 暴露策略，结合下一代测序（NGS）和 qRT-PCR 以及生物信息学和计算分析，比较了幼稚和 LPS 暴露的七鳃鳗整个咽转录组，首次提供了 LPS 诱导造血器官炎症反应中细胞色素基因表达和 miRNA 调控的全貌。在 LPS 诱导的炎症反应中，2B、2C、2J、2U、4B 和 4F 亚家族的细胞色素被下调，miRNA 网络相互作用表明不同保守和种特异性的 miRNA 参与细胞色素基因的转录后调控，并且在七鳃鳗的炎症反应中，可能存在特定 miRNA 调节炎症和细胞色素分子之间的相互作用。
