Chen Lingqiao, Wang Haoyu, Yang Junhua, Yang Xianli, Zhang Mengyuan, Zhao Zhihui, Fan Yingying, Wang Cheng, Wang Jianhua
College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China.
Shanghai Key Laboratory of Protected Horticultural Technology, Laboratory of Quality and Safety Risk Assessment for Agro-Products (Shanghai), Institute for Agro-Food Standards and Testing Technology, Ministry of Agriculture, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Shanghai 201403, China.
J Fungi (Basel). 2021 Oct 16;7(10):871. doi: 10.3390/jof7100871.
Fusarium blight of wheat is usually caused by , and the pathogenic fungi will secrete effectors into the host plant tissue to affect its normal physiological process, so as to make it pathogenic. The CFEM (Common in Fungal Extracellular Membrane) protein domain is unique to fungi, but it is not found in all fungi. The CFEM protein contained in may be closely related to pathogenicity. In this study, 23 FgCFEM proteins were identified from the genome. Then, features of these proteins, such as signal peptide, subcellular localization, and transmembrane domains, etc., were analyzed and candidate effectors were screened out. Sequence alignment results revealed that each FgCFEM protein contains one CFEM domain. The amino acids of the CFEM domain are highly conserved and contain eight spaced cysteines, with the exception that FgCFEM8, 9, and 15 lack two cysteines and three cysteines were missed in FgCFEM18 and FgCFEM22. A recently identified CFEM_DR motif was detected in 11 FgCFEMs, and importantly we identified two new conserved motifs containing about 29 and 18 amino acids (CFEM_WR and CFEM_KF), respectively, in some of FgCFEM proteins. Transcriptome analysis of the genes encoding CFEM proteins indicated that all the CFEM-containing genes were expressed during wheat infection, with seven and six genes significantly up- and down-regulated, respectively, compared with in planta and in vitro. Based on the above analysis, FgCFEM11 and FgCFEM23 were predicted to be effectors. This study provides the basis for future functional analyses of CFEM proteins in .
小麦赤霉病通常由[病原菌名称未给出]引起，致病真菌会向寄主植物组织中分泌效应蛋白以影响其正常生理过程，从而使其致病。CFEM（常见于真菌细胞外膜）蛋白结构域是真菌所特有的，但并非在所有真菌中都能找到。[未提及的真菌名称]中含有的CFEM蛋白可能与致病性密切相关。在本研究中，从[未提及的真菌名称]基因组中鉴定出了23个FgCFEM蛋白。然后，分析了这些蛋白的信号肽、亚细胞定位和跨膜结构域等特征，并筛选出候选效应蛋白。序列比对结果显示，每个FgCFEM蛋白都包含一个CFEM结构域。CFEM结构域的氨基酸高度保守，含有8个间隔排列的半胱氨酸，不过FgCFEM8、9和15缺少两个半胱氨酸，FgCFEM18和FgCFEM22中则缺失了3个半胱氨酸。在11个FgCFEM蛋白中检测到了一个最近鉴定出的CFEM_DR基序，重要的是，我们在一些FgCFEM蛋白中分别鉴定出了两个新的保守基序，分别包含约29个和18个氨基酸（CFEM_WR和CFEM_KF）。对编码CFEM蛋白的基因进行转录组分析表明，所有含CFEM的基因在小麦感染过程中均有表达，与在植物体内和体外相比，分别有7个和6个基因显著上调和下调。基于上述分析，预测FgCFEM11和FgCFEM23为效应蛋白。本研究为今后对[未提及的真菌名称]中CFEM蛋白的功能分析提供了依据。
