Sreejith Kamalalayam Rajan, Umer Muhammad, Dirr Larissa, Bailly Benjamin, Guillon Patrice, von Itzstein Mark, Soda Narshone, Kasetsirikul Surasak, Shiddiky Muhammad J A, Nguyen Nam-Trung
Queensland Micro- and Nanotechnology Centre, Griffith University, 170 Kessels Road, Nathan, QLD 4111, Australia.
Institute for Glycomics, Gold Coast Campus, Griffith University, 1 Parklands Drive, Southport, QLD 4222, Australia.
Micromachines (Basel). 2021 Sep 24;12(10):1151. doi: 10.3390/mi12101151.
This paper reports the design, development, and testing of a novel, yet simple and low-cost portable device for the rapid detection of SARS-CoV-2. The device performs loop mediated isothermal amplification (LAMP) and provides visually distinguishable images of the fluorescence emitted from the samples. The device utilises an aluminium block embedded with a cartridge heater for isothermal heating of the sample and a single-board computer and camera for fluorescence detection. The device demonstrates promising results within 20 min using clinically relevant starting concentrations of the synthetic template. Time-to-signal data for this device are considerably lower compared to standard quantitative Polymerase Chain Reaction(qPCR) machine (~10-20 min vs. >38 min) for 1 × 10 starting template copy number. The device in its fully optimized and characterized state can potentially be used as simple to operate, rapid, sensitive, and inexpensive platform for population screening as well as point-of-need severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) detection and patient management.
本文报道了一种新型、简单且低成本的便携式设备的设计、开发和测试，该设备用于快速检测新型冠状病毒2（SARS-CoV-2）。该设备进行环介导等温扩增（LAMP），并提供从样品发出的荧光的视觉上可区分的图像。该设备利用嵌入有筒式加热器的铝块对样品进行等温加热，并利用单板计算机和相机进行荧光检测。使用临床相关的合成模板起始浓度，该设备在20分钟内显示出有前景的结果。对于1×10起始模板拷贝数，该设备的信号产生时间数据与标准定量聚合酶链反应（qPCR）机器相比要低得多（约10 - 20分钟对>38分钟）。处于完全优化和表征状态的该设备有可能用作操作简单、快速、灵敏且廉价的平台，用于人群筛查以及即时需求的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）检测和患者管理。
