Li Yongli, Wu Mei X
Wellman Center for Photomedicine, Massachusetts General Hospital, Department of Dermatology, Harvard Medical School, 50 Blossom Street, Boston, MA 02114, USA.
Microorganisms. 2021 Oct 1;9(10):2074. doi: 10.3390/microorganisms9102074.
Chronic wound infections are often caused by multi-species biofilms and these biofilm-embedded bacteria exhibit remarkable tolerance to existing antibiotics, which presents huge challenges to control such infections in the wounds. In this investigation, we established a polymicrobial biofilm composed of , , , and . We tested a cocktail therapy comprising 405-nm blue light (BL), carvacrol (Ca), and antibiotics on the multispecies biofilm. Despite the fact that all strains used to form the biofilm were susceptible to ciprofloxacin (CIP) in planktonic cultures, the biofilm was found to withstand ciprofloxacin as well as BL-Ca dual treatment, mainly because outgrew and became dominant in the biofilm after each treatment. Strikingly, when ciprofloxacin was combined with BL-Ca, the multispecies biofilms succumbed substantially and were eradicated at an efficacy of 99.9%. Mechanistically, BL-Ca treatment increased membrane permeability and potentiated the anti-biofilm activity of ciprofloxacin, probably by facilitating ciprofloxacin's entrance of the bacteria, which is particularly significant for , a species that is refractory to either ciprofloxacin or BL-Ca dual treatment. The results suggest that bacterial membrane damage can be one of the pivotal strategies to subvert biofilm tolerance and combat the recalcitrant multispecies biofilms.
慢性伤口感染通常由多种细菌形成的生物膜引起，这些嵌入生物膜的细菌对现有抗生素表现出显著的耐受性，这给控制伤口感染带来了巨大挑战。在本研究中，我们建立了一种由[具体细菌种类1]、[具体细菌种类2]、[具体细菌种类3]和[具体细菌种类4]组成的多菌种生物膜。我们测试了一种包含405纳米蓝光（BL）、香芹酚（Ca）和抗生素的联合疗法对该多菌种生物膜的作用。尽管用于形成生物膜的所有菌株在浮游培养中对环丙沙星（CIP）敏感，但发现该生物膜能够抵抗环丙沙星以及蓝光 - 香芹酚的双重处理，主要原因是在每次处理后[具体细菌种类]在生物膜中生长并占主导地位。令人惊讶的是，当环丙沙星与蓝光 - 香芹酚联合使用时，多菌种生物膜大量死亡并以99.9%的效率被根除。从机制上讲，蓝光 - 香芹酚处理增加了膜通透性并增强了环丙沙星的抗生物膜活性，可能是通过促进环丙沙星进入细菌，这对于[具体细菌种类]尤为重要，该菌种对环丙沙星或蓝光 - 香芹酚双重处理均具有抗性。结果表明，细菌膜损伤可能是颠覆生物膜耐受性和对抗顽固的多菌种生物膜的关键策略之一。
