Prikhod'ko G G, Babkin I V
Genetika. 1991 Jan;27(1):13-26.
HindIII-O/N DNA fragments of vaccinia virus (VV) of the LIVP strain were mapped using thirteen restriction endonucleases. Nucleotide sequences of the HindIII-O fragment (1530 bp) as well as of a site of the HindIII-N genome fragment 353 bp in size were determined. Comparison of restriction maps and nucleotide sequences of VV strains (WR and LIVP) demonstrated that DNA of VV LIVP contained % deletions and 2 insertions. "Reliable" short direct repeats were localized and their possible role in formation of DNA deletions was shown. It was suggested that VV endonuclease and DNA-ligase participate in replication and repair processes. Mechanism of formation of variable sequences of viral genomes is discussed.
使用13种限制性内切酶对LIVP株痘苗病毒（VV）的HindIII - O/N DNA片段进行了图谱绘制。测定了HindIII - O片段（1530 bp）以及大小为353 bp的HindIII - N基因组片段一个位点的核苷酸序列。对VV毒株（WR和LIVP）的限制性图谱和核苷酸序列进行比较表明，VV LIVP的DNA存在%的缺失和2处插入。定位了“可靠的”短直接重复序列，并显示了它们在DNA缺失形成中的可能作用。有人提出VV内切酶和DNA连接酶参与复制和修复过程。讨论了病毒基因组可变序列的形成机制。
