Goelen Jan, Alexander Benoni, Wijesinghe Haren Eranga, Evans Emily, Pawar Gopal, Horniblow Richard D, Batchelor Hannah K
School of Pharmacy, Institute of Clinical Science, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
School of Biomedical Science, Institute of Clinical Science, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Pharmaceutics. 2021 Oct 19;13(10):1729. doi: 10.3390/pharmaceutics13101729.
Previous studies have used magnetic resonance imaging (MRI) to quantify the fluid in the stomach and small intestine of children, and the stomach, small intestine and colon of adults. This is the first study to quantify fluid volumes and distribution using MRI in the paediatric colon. MRI datasets from 28 fasted (aged 0-15 years) and 18 fluid-fed (aged 10-16 years) paediatric participants were acquired during routine clinical care. A series of 2D- and 3D-based software protocols were used to measure colonic fluid volume and localisation. The paediatric colon contained a mean volume of 22.5 mL ± 41.3 mL fluid, (range 0-167.5 mL, median volume 0.80 mL) in 15.5 ± 17.5 discreet fluid pockets (median 12). The proportion of the fluid pockets larger than 1 mL was 9.6%, which contributed to 94.5% of the total fluid volume observed. No correlation was detected between all-ages and colonic fluid volume, nor was a difference in colonic fluid volumes observed based on sex, fed state or age group based on ICH-classifications. This study quantified fluid volumes within the paediatric colon, and these data will aid and accelerate the development of biorelevant tools to progress paediatric drug development for colon-targeting formulations.
以往的研究使用磁共振成像（MRI）对儿童的胃和小肠以及成人的胃、小肠和结肠中的液体进行了量化。这是第一项使用MRI对小儿结肠中的液体体积和分布进行量化的研究。在常规临床护理期间，采集了28名禁食（年龄0 - 15岁）和18名喂食液体（年龄10 - 16岁）的儿科参与者的MRI数据集。使用了一系列基于二维和三维的软件协议来测量结肠液体体积和定位。小儿结肠中平均含有22.5 mL±41.3 mL液体（范围0 - 167.5 mL，中位数体积0.80 mL），分布在15.5±17.5个离散的液囊中（中位数为12个）。大于1 mL的液囊比例为9.6%，其占观察到的总液体体积的94.5%。未检测到全年龄段与结肠液体体积之间的相关性，也未观察到基于性别、喂食状态或基于ICH分类的年龄组的结肠液体体积差异。本研究对小儿结肠内的液体体积进行了量化，这些数据将有助于并加速生物相关工具的开发，以推进用于结肠靶向制剂的儿科药物研发。
