Graduate School of Sport Sciences, Waseda University, Tokorozawa 359-1192, Japan.
Faculty of Sport Sciences, Waseda University, Tokorozawa 359-1192, Japan.
Medicina (Kaunas). 2021 Sep 27;57(10):1028. doi: 10.3390/medicina57101028.
: The purpose of this study was to investigate the influences of oral high-dose genistein (GE) administration on exercise-induced oxidative stress, inflammatory response and tissue damage. : Thirty-two mice were randomly divided into control group (Con; sedentary/0.5% CMC-Na), GE administrated group (GE; sedentary/GE dosed), exercise group (Ex; exercise/0.5% CMC-Na), or GE administrated plus exercise group (GE + Ex; exercise/GE dosed), mice in the GE and GE + Ex group were given GE orally at the dose of 200 mg/kg weight. : Plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT) levels, liver interleukin (IL)-6, IL-1β, superoxide dismutase 1 (), catalase (), hemeoxygenase-1 () gene expression levels and skeletal muscle IL-6, nuclear factor erythroid 2-related factor (), and gene expression levels increased immediately after exhaustive exercise. GE supplementation increased liver protein carbonyl concentrations. On the other hand, GE supplementation significantly decreased , gene expression levels in the liver and , and gene expression levels in the skeletal muscles. : Acute exercise induced organ damage, inflammation, and oxidative stress in skeletal muscles and the liver. However, a single dose of GE supplementation before exercise did not lead to favorable antioxidant and anti-inflammatory effects in this study.
: 本研究旨在探讨口服高剂量金雀异黄素（GE）对运动诱导的氧化应激、炎症反应和组织损伤的影响。 : 32 只小鼠随机分为对照组（Con；静息/0.5% CMC-Na）、GE 给药组（GE；静息/GE 给药）、运动组（Ex；运动/0.5% CMC-Na）或 GE 给药加运动组（GE + Ex；运动/GE 给药），GE 和 GE + Ex 组小鼠口服给予 200mg/kg 体重的 GE。 : 运动后即刻，血浆天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）水平、肝脏白细胞介素（IL）-6、IL-1β、超氧化物歧化酶 1（）、过氧化氢酶（）、血红素加氧酶-1（）基因表达水平以及骨骼肌白细胞介素（IL）-6、核因子红细胞 2 相关因子（）和基因表达水平均升高。GE 补充剂增加了肝脏蛋白羰基浓度。另一方面，GE 补充剂显著降低了肝脏和骨骼肌中的、基因表达水平。 : 急性运动导致肌肉和肝脏的器官损伤、炎症和氧化应激。然而，在本研究中，运动前单次给予 GE 补充剂并没有产生有利的抗氧化和抗炎作用。
