Rheumatology/Immunology and Allergy, Department of Medicine, Tri-Service General Hospital, Department of Orthopedics, Tri-Service General Hospital, Taipei 114, Taiwan.
Department of Orthopedics, Tri-Service General Hospital, Taipei, Department of Orthopedics, Tri-Service General Hospital, Taipei 114, Taiwan.
Nutrients. 2019 May 27;11(5):1180. doi: 10.3390/nu11051180.
Genistein is an isoflavone extracted from soybean (Glycine max). This compound has anti-inflammatory, anti-oxidative, and anti-cancer effects; however, the mechanism underlying the effects of genistein on IL-1β-stimulated human osteoarthritis (OA) chondrocytes remains unknown. Our objectives in this study were to explore the anti-inflammatory effects of genistein on IL-1β-stimulated human OA chondrocytes and to investigate the potential mechanisms which underlie them. Our results from an in-vitro model of osteoarthritis indicate that genistein inhibits the IL-1β-induced expression of the catabolic factors nitric oxide synthase 2 (NOS2), cyclooxygenase 2 (COX-2), and matrix metalloproteinases (MMPs). Genistein was shown to stimulate Ho-1 expression, which has been associated with Nrf-2 pathway activation in human chondrocytes. In a rat model, genistein was also shown to attenuate the progression of traumatic osteoarthritis. Taken together, these results demonstrate the effectiveness of genistein in mediating the inflammation associated with joint disorders. Our results also indicate that genistein could potentially serve as an alternative therapeutic treatment for OA.
染料木黄酮是从大豆（Glycine max）中提取的异黄酮。这种化合物具有抗炎、抗氧化和抗癌作用；然而，染料木黄酮对白细胞介素-1β刺激的人骨关节炎（OA）软骨细胞的作用机制尚不清楚。我们在这项研究中的目的是探讨染料木黄酮对白细胞介素-1β刺激的人 OA 软骨细胞的抗炎作用，并研究其潜在机制。我们在骨关节炎的体外模型中的结果表明，染料木黄酮抑制白细胞介素-1β诱导的一氧化氮合酶 2（NOS2）、环氧化酶 2（COX-2）和基质金属蛋白酶（MMPs）的分解代谢因子的表达。染料木黄酮被证明能刺激 Ho-1 的表达，这与人类软骨细胞中 Nrf-2 途径的激活有关。在大鼠模型中，染料木黄酮也能减轻创伤性骨关节炎的进展。综上所述，这些结果表明染料木黄酮在介导与关节疾病相关的炎症方面是有效的。我们的结果还表明，染料木黄酮可能作为 OA 的一种替代治疗方法。
