Functional Foods and Nutraceuticals Unit, Department of Medical Biochemistry, Afe Babalola University, P.M.B. 5454, Ado Ekiti 360001, Nigeria.
Functional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology, P.M.B. 704, Akure 340001, Nigeria.
Nutrients. 2021 Oct 8;13(10):3529. doi: 10.3390/nu13103529.
In hypertensive individuals, platelet morphology and function have been discovered to be altered, and this has been linked to the development of vascular disease, including erectile dysfunction (ED). The impact of nutritional supplementation with (tiger nut, TN) and (walnut, WN) on androgen levels, ectonucleotidases, and adenosine deaminase (ADA) activities in platelets from L-NAME (Nω-nitro-L-arginine methyl ester hydrochloride) challenged rats were investigated. We hypothesized that these nuts may show a protective effect on platelets aggregation and possibly enhance the sex hormones, thereby reverting vasoconstriction. rats (male; 250-300 g; = 10) were grouped into seven groups as follows: basal diet control group (I); basal diet/L-NAME/Viagra (5 mg/kg/day) as positive control group (II); ED-induced group (basal diet/L-NAME) (III); diet supplemented processed TN (20%)/L-NAME (IV); diet supplemented raw TN (20%)/L-NAME (V); diet supplemented processed WN (20%)/L-NAME (VI); and diet supplemented raw WN (20%)/L-NAME (VII). The rats were given their regular diet for 2 weeks prior to actually receiving L-NAME (40 mg/kg/day) for ten days to induce hypertension. Platelet androgen levels, ectonucleotidases, and ADA were all measured. L-NAME considerably lowers testosterone levels (54.5 ± 2.2; < 0.05). Supplementing the TN and WN diets revealed improved testosterone levels as compared to the control (306.7 ± 5.7), but luteinizing hormone levels remained unchanged. Compared to control groups, the L-NAME-treated group showed a rise in ATP (127.5%) hydrolysis and ADA (116.7%) activity, and also a decrease in ADP (76%) and AMP (45%) hydrolysis. Both TN and WN supplemented diets resulted in substantial ( < 0.05) reversal effects. Enhanced testosterone levels and modulation of the purinergic system in platelets by TN and WN could be one of the mechanisms by which they aid in vasoconstriction control.
在高血压个体中，已经发现血小板形态和功能发生改变，这与血管疾病的发展有关，包括勃起功能障碍（ED）。本研究旨在探讨营养补充 （虎坚果，TN） 和 （胡桃，WN） 对 L-NAME（Nω-硝基-L-精氨酸甲酯盐酸盐）诱导的大鼠血小板中雄激素水平、外核苷酸酶和腺苷脱氨酶（ADA）活性的影响。我们假设这些坚果可能对血小板聚集有保护作用，并可能增强性激素，从而逆转血管收缩。雄性大鼠（250-300g，n=10）被分为 7 组：基础饮食对照组（I）；基础饮食/L-NAME/伟哥（5mg/kg/天）作为阳性对照组（II）；ED 诱导组（基础饮食/L-NAME）（III）；补充加工 TN（20%）/L-NAME 组（IV）；补充生 TN（20%）/L-NAME 组（V）；补充加工 WN（20%）/L-NAME 组（VI）；补充生 WN（20%）/L-NAME 组（VII）。在实际给予 L-NAME（40mg/kg/天）10 天以诱导高血压之前，大鼠先给予常规饮食 2 周。测量血小板雄激素水平、外核苷酸酶和 ADA。L-NAME 显著降低睾酮水平（54.5±2.2；<0.05）。与对照组相比，补充 TN 和 WN 饮食可提高睾酮水平（306.7±5.7），但黄体生成素水平保持不变。与对照组相比，L-NAME 处理组的 ATP（127.5%）水解和 ADA（116.7%）活性增加，ADP（76%）和 AMP（45%）水解减少。补充 TN 和 WN 的饮食都有显著的（<0.05）逆转作用。TN 和 WN 增强的睾酮水平和血小板嘌呤能系统的调节可能是它们有助于控制血管收缩的机制之一。
