School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian 223003, China.
College of Food Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 250003, China.
Molecules. 2021 Oct 19;26(20):6309. doi: 10.3390/molecules26206309.
Fengycin, as a lipopeptide produced by , displays potent activity against filamentous fungi, including and , which exhibits a wide range of potential applications in food industries, agriculture, and medicine. To better clarify the regulatory mechanism of fructose on fengycin biosynthesis, the iTRAQ-based proteomic analysis was utilized to investigate the differentially expressed proteins of fmb-60 cultivated in ML (without fructose) and MLF (with fructose) medium. The results indicated that a total of 811 proteins, including 248 proteins with differential expression levels (162 which were upregulated (fold > 2) and 86, which were downregulated (fold < 0.5) were detected, and most of the proteins are associated with cellular metabolism, biosynthesis, and biological regulation process. Moreover, the target genes' relative expression was conducted using quantitative real-time PCR to validate the proteomic analysis results. Based on the results of proteome analysis, the supposed pathways of fructose enhancing fengycin biosynthesis in fmb-60 can be summarized as improvement of the metabolic process, including cellular amino acid and amide, fatty acid biosynthesis, peptide and protein, nucleotide and nucleobase-containing compound, drug/toxin, cofactor, and vitamin; reinforcement of peptide/protein translation, modification, biological process, and response to a stimulus. In conclusion, this study represents a comprehensive and systematic investigation of the fructose mechanism on improving fengycin biosynthesis in , which will provide a road map to facilitate the potential application of fengycin or its homolog in defending against filamentous fungi.
丰原菌素是由 产生的一种脂肽，对丝状真菌具有很强的活性，包括 和 ，在食品工业、农业和医学中有广泛的潜在应用。为了更好地阐明果糖对丰原菌素生物合成的调控机制，利用 iTRAQ 基于蛋白质组学分析研究了在 ML（无果糖）和 MLF（有果糖）培养基中培养的 fmb-60 的差异表达蛋白。结果表明，共检测到 811 种蛋白质，包括 248 种差异表达水平的蛋白质（162 种上调（倍数>2）和 86 种下调（倍数<0.5），大多数蛋白质与细胞代谢、生物合成和生物调节过程有关。此外，还使用定量实时 PCR 对目标基因的相对表达进行了检测，以验证蛋白质组学分析结果。基于蛋白质组分析的结果，可以总结出果糖增强 fmb-60 中丰原菌素生物合成的假设途径，包括改善代谢过程，包括细胞氨基酸和酰胺、脂肪酸生物合成、肽和蛋白质、核苷酸和核苷碱基化合物、药物/毒素、辅因子和维生素；加强肽/蛋白质翻译、修饰、生物过程和对刺激的反应。总之，本研究代表了对 中果糖提高丰原菌素生物合成机制的全面和系统研究，为丰原菌素或其同系物在防治丝状真菌方面的潜在应用提供了路线图。
