School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian 223003, China.
National Engineering Research Center for Functional Food, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Genes (Basel). 2022 May 31;13(6):984. doi: 10.3390/genes13060984.
Fengycin is a lipopeptide produced by that has a strong inhibitory effect on filamentous fungi; however, its use is restricted due to poor production and low yield. Previous studies have shown that fengycin biosynthesis in was found to be significantly increased after fructose addition. This study investigated the effect of fructose on fengycin production and its regulation mechanism in by transcriptome sequencing. According to the RNA sequencing data, 458 genes were upregulated and 879 genes were downregulated. Transcriptome analysis results showed that fructose changed the transcription of amino acid synthesis, fatty acid metabolism, and energy metabolism; alterations in these metabolic pathways contribute to the synthesis of fengycin. In an MLF medium (modified Landy medium with fructose), the expression level of the fengycin operon was two-times higher than in an ML medium (modified Landy medium). After fructose was added to , the fengycin-synthesis-associated genes were activated in the process of fengycin synthesis.
丰原菌素是一种由 产生的脂肽，对丝状真菌具有很强的抑制作用；然而，由于产量低、生产困难，其应用受到限制。先前的研究表明，添加果糖后， 中丰原菌素的生物合成显著增加。本研究通过转录组测序，探讨了果糖对 的丰原菌素生产及其调控机制的影响。根据 RNA 测序数据，有 458 个基因上调，879 个基因下调。转录组分析结果表明，果糖改变了氨基酸合成、脂肪酸代谢和能量代谢的转录；这些代谢途径的改变有助于丰原菌素的合成。在 MLF 培养基（含果糖的改良 Landy 培养基）中，丰原菌素操纵子的表达水平比在 ML 培养基（改良 Landy 培养基）中高两倍。向 中添加果糖后，丰原菌素合成相关基因在丰原菌素合成过程中被激活。
