Boorboori Mohammad Reza, Li Zhou, Yan Xue, Dan Mu, Zhang Zhixing, Lin Wenxiong, Fang Changxun
Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Plants (Basel). 2021 Oct 18;10(10):2210. doi: 10.3390/plants10102210.
Arsenic is one of the most hazardous metalloids in nature, and due to its high water solubility, it is one of the most important causes of pollution. However, silicon reduces the uptake and transport of arsenic in rice. This study investigates the interaction of different arsenic and silicon levels on dry weight, protein content, and concentrations of arsenic and silicon in two different rice shoots and roots of Dular wild-type (DU-WT) and Dular Lsi1-overexpressed (DU-OE) rice. It should be noted that all seedlings were subjected to four different treatments. For RNA-seq and qPCR, the DU-WT genotype was selected as the control and DU-OE as the treatment. With the addition of silicone treatment, dry weight and protein content in the shoots and roots of both rice lines were increased, while the concentration of arsenic in these two organs was decreased. When seedlings were exposed to arsenic treatments, protein content, silicon concentration, and dry weight were decreased in both roots and shoots, while arsenic concentration was increased in both rice genotypes. The RNA-seq in DU-OE showed 5823 differentially expressed genes (DEGs), of which 2604 were up-regulated and 3219 down-regulated. Treatment of rice by arsenic and silicon has changed the expression of genes encoding cytokinin-responsive GATA transcription factor 1, protein IN2-1 homolog B, calcium-binding EGF domain-containing protein, Os01g0369700 protein, probable glutathione S-transferase GSTU1, glutathione S-transferase protein, Os09g0367700 protein, isocitrate dehydrogenase (NADP), and Os08g0522400 protein in the root of DU-OE. The present study's findings showed that in the presence of silicon, the transgenic genotype is much more resistant to arsenic than the wild genotype of Dular rice.
砷是自然界中危害最大的类金属之一，由于其在水中的高溶解度，它是最重要的污染原因之一。然而，硅会降低水稻对砷的吸收和运输。本研究调查了不同砷和硅水平对两种不同水稻（杜拉野生型（DU-WT）和杜拉Lsi1过表达型（DU-OE））地上部和根部干重、蛋白质含量以及砷和硅浓度的相互作用。需要注意的是，所有幼苗都接受了四种不同的处理。对于RNA测序和定量聚合酶链反应（qPCR），选择DU-WT基因型作为对照，DU-OE作为处理组。添加硅处理后，两个水稻品系地上部和根部的干重和蛋白质含量均增加，而这两个器官中的砷浓度降低。当幼苗暴露于砷处理时，两个水稻基因型的根部和地上部的蛋白质含量、硅浓度和干重均降低，而砷浓度增加。DU-OE中的RNA测序显示有5823个差异表达基因（DEG），其中2604个上调，3219个下调。砷和硅对水稻处理改变了DU-OE根部编码细胞分裂素响应型GATA转录因子1、蛋白质IN2-1同源物B、含钙结合表皮生长因子结构域蛋白、Os01g0369700蛋白、可能的谷胱甘肽S-转移酶GSTU1、谷胱甘肽S-转移酶蛋白、Os09g0367700蛋白、异柠檬酸脱氢酶（NADP）和Os08g0522400蛋白的基因表达。本研究结果表明，在硅存在的情况下，转基因基因型比杜拉水稻的野生基因型对砷的抗性更强。
