Ramlagan Piteesha, Issa Marwa Yousry, Rondeau Philippe, Bourdon Emmanuel, Bahorun Theeshan, Farag Mohamed A, Neergheen Vidushi S
Biopharmaceutical Unit, Centre for Biomedical and Biomaterials Research, MSIRI Building, University of Mauritius, Réduit, Mauritius.
Department of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, Réduit, Mauritius.
J Am Nutr Assoc. 2023 Jan;42(1):36-54. doi: 10.1080/07315724.2021.1978349. Epub 2021 Oct 22.
It was aimed at determining which polyphenolic compound(s) in pomegranate mesocarp extract (PME) is liable for the antioxidant, anti-glycation and anti-CD36 activities.
The PME was fractionated using liquid-liquid extraction method. The fractions were tested for their polyphenolic content, antioxidant potency, anti-glycation activity and anti-CD36 potential. The metabolite compositions of PME and derived fractions were investigated in an untargeted manner using metabolomics in relation to its antioxidant and anti-glycation activities.
The ethyl acetate and -butanol fractions of the pomegranate mesocarp demonstrated highest antioxidant and anti-glycation potencies. These fractions, represented by gallic and ellagic acids monomers, were enriched in tannins and phenolic acids. Orthogonal partial least squares discriminate analysis (OPLS-DA) modeling of ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) metabolite profiles from the different pomegranate mesocarp fractions indicated that gallic and ellagic acids were potential contributors to the antioxidant and anti-glycation effects of the pomegranate mesocarp. At cellular level, the polyphenolic-rich crude extract as well as the ethyl acetate, -butanol and aqueous residual fractions suppressed the protein expression of CD36. The anti-CD36 activity of these extracts and fractions was attributed to the presence of punicalagin, the ellagitannins that occurred in equal amount in the different fractions.
This work demonstrated the protective effect of the non-edible part of the pomegranate fruit and showed that gallic and ellagic acids account for the antioxidant and anti-glycation activities while punicalagin is liable for the anti-CD36 activity of PME.
旨在确定石榴中果皮提取物（PME）中的哪种多酚化合物具有抗氧化、抗糖化和抗CD36活性。
采用液-液萃取法对PME进行分离。对分离出的各部分进行多酚含量、抗氧化能力、抗糖化活性和抗CD36潜力的检测。利用代谢组学以非靶向方式研究PME及其衍生部分的代谢物组成与其抗氧化和抗糖化活性的关系。
石榴中果皮的乙酸乙酯和正丁醇部分表现出最高的抗氧化和抗糖化能力。这些部分以没食子酸和鞣花酸单体为代表，富含单宁和酚酸。对不同石榴中果皮部分的超高效液相色谱-质谱（UPLC-MS）代谢物谱进行正交偏最小二乘法判别分析（OPLS-DA）建模，结果表明没食子酸和鞣花酸是石榴中果皮抗氧化和抗糖化作用的潜在贡献者。在细胞水平上，富含多酚的粗提物以及乙酸乙酯、正丁醇和水相残余部分均抑制了CD36的蛋白表达。这些提取物和部分的抗CD36活性归因于石榴皮素的存在，石榴皮素是一种鞣花单宁，在不同部分中含量相等。
这项研究证明了石榴果实不可食用部分的保护作用，并表明没食子酸和鞣花酸具有抗氧化和抗糖化活性，而石榴皮素是PME抗CD36活性的原因。
