College of Animal Science and Technology, Anhui Agricultural University, No. 130 Changjiang West Road, Hefei, 230036, People's Republic of China.
Department of Microbiology, Abbottabad University of Science and Technology, Havelian Abbottabad, Pakistan.
Trop Anim Health Prod. 2022 Jul 9;54(4):228. doi: 10.1007/s11250-022-03222-7.
In the present study, we determined the potential effects of ellagic acid and mesocarp extract of Punica granatum on the productive and reproduction performance of laying hens. Five treatment groups were setup: (1) control group (without ellagic acid), (2) 50 mg of ellagic acid, (3) 100 mg of ellagic acid, (4) 200 mg of ellagic acid, and (5) mesocarp extract of P. granatum. All the groups were investigated for feed intake, body weight, egg production, egg quality, fertility, hatchability, antioxidant status of serum and liver, lipid peroxidation, and antibacterial activities. Egg production, feed intake, and bodyweight were significantly increased (p < 0.05) with 100 mg of ellagic acid and P. granatum extract while no significant effect was observed on albumen and yolk weight, yolk index, yolk color, egg-shape index, and Haugh unit. Both ellagic acid and P. granatum extract significantly improved hatchability while 100 and 200 mg/kg of ellagic acid numerically decreased fertility. Besides, ellagic acid (100 mg/kg) and P. granatum extract significantly decreased malondialdehyde concentration and increased total antioxidant capacity, glutathione peroxidase, and total superoxide dismutase in serum and liver samples of laying hens (p < 0.05). The lipid peroxidation was decreased among the treatment groups, with 100 mg of ellagic acid and P. granatum extract showed the best activity. Moreover, ellagic acid demonstrated strong killing activity against Escherichia coli and Staphylococcus aureus while it was ineffective against methicillin-resistant S. aureus. Our results conclude that ellagic acid and P. granatum promoted egg production, hatchability, and antioxidant enzyme activities of the laying hens.
在本研究中，我们确定了鞣花酸和石榴中果皮提取物对产蛋鸡生产性能和繁殖性能的潜在影响。设置了 5 个处理组：（1）对照组（不含鞣花酸）、（2）50mg 鞣花酸、（3）100mg 鞣花酸、（4）200mg 鞣花酸和（5）石榴中果皮提取物。所有组均进行了饲料摄入量、体重、产蛋量、蛋品质、受精率、孵化率、血清和肝脏抗氧化状态、脂质过氧化、抗菌活性的研究。100mg 鞣花酸和石榴中果皮提取物可显著提高产蛋量、饲料摄入量和体重（p<0.05），而对蛋白和蛋黄重量、蛋黄指数、蛋黄颜色、蛋形指数和哈夫单位没有显著影响。鞣花酸和石榴中果皮提取物均显著提高了孵化率，而 100 和 200mg/kg 鞣花酸则使受精率呈数值下降趋势。此外，鞣花酸（100mg/kg）和石榴中果皮提取物可显著降低血清和肝脏样品中的丙二醛浓度，增加总抗氧化能力、谷胱甘肽过氧化物酶和总超氧化物歧化酶（p<0.05）。处理组的脂质过氧化减少，100mg 鞣花酸和石榴中果皮提取物的活性最强。此外，鞣花酸对大肠杆菌和金黄色葡萄球菌表现出较强的杀菌活性，但对耐甲氧西林金黄色葡萄球菌无效。我们的结果表明，鞣花酸和石榴可促进产蛋鸡的产蛋量、孵化率和抗氧化酶活性。
