Hacettepe University, Graduate School of Science and Engineering, Bioengineering Division, Ankara, Turkey.
Hacettepe University, Graduate School of Science and Engineering, Bioengineering Division, Ankara, Turkey.
Int J Biol Macromol. 2021 Dec 15;193(Pt A):276-286. doi: 10.1016/j.ijbiomac.2021.10.104. Epub 2021 Oct 24.
In this work we investigated tenogenic differentiation of adipose-derived mesenchymal stem cells (AdMSCs), which were seeded onto silk fibroin/poly-3-hydroxybutyrate (SF/P3HB) scaffolds with aligned topography, and high mechanical strength. The electrospinning process was optimized by using the response surface method (RSM) and SF/P3HB nanofibrous matrices with a total polymer concentration of 5% (SF: PHB = 3: 1), flow rate 1 mL/h, collector rotation speed 2000 rpm, applied voltage 14 kV, and collector distance 25 cm were obtained. The average fiber diameter was 699 ± 203 nm and 80% of the nanofibers were aligned within the ±15 range. SF reinforcement reduced the crystallinity of P3HB, and the elastic modulus was found to be 197.0 ± 7.7 MPa. The scaffolds showed bacteriostatic effect. A 21-day of cell culture study was performed with rat rAdMSCs in the absence and presence of tenogenic differentiation factor-5 (GDF-5). The results demonstrated that SF/P3HB scaffolds allow the cells to proliferate and differentiate to the tenocytes. However, no significant effect of GDF-5 on the differentiation of cells was observed. These findings indicated that our aligned SF/P3HB scaffolds have a significant potential to be used for tendon tissue engineering.
在这项工作中，我们研究了脂肪间充质干细胞（AdMSCs）的腱向分化，将其接种到具有定向形貌和高机械强度的丝素/聚 3-羟基丁酸（SF/P3HB）支架上。通过使用响应面法（RSM）优化了电纺工艺，得到了总聚合物浓度为 5%（SF:PHB=3:1）、流速为 1mL/h、收集器转速为 2000rpm、施加电压为 14kV 和收集器距离为 25cm 的 SF/P3HB 纳米纤维基质。平均纤维直径为 699±203nm，80%的纳米纤维在±15 范围内定向排列。SF 增强降低了 P3HB 的结晶度，弹性模量为 197.0±7.7MPa。支架具有抑菌作用。在不存在和存在腱形成因子-5（GDF-5）的情况下，用大鼠 rAdMSCs 进行了 21 天的细胞培养研究。结果表明，SF/P3HB 支架允许细胞增殖并向腱细胞分化。然而，没有观察到 GDF-5 对细胞分化的显著影响。这些发现表明，我们的定向 SF/P3HB 支架具有很大的潜力可用于肌腱组织工程。
