Lahtiperä S, Naukkarinen A, Collan Y
Arch Toxicol Suppl. 1986;9:315-9. doi: 10.1007/978-3-642-71248-7_54.
The effects of a poisonous mushroom, Cortinarius speciosissimus, on rat kidney were studied by transmission electron microscopy. Suspension of dried mushroom (500 mg/kg body weight) was administered as a single dose directly into the esophagus. The kidneys were fixed by perfusion, and both cortex and medulla were sampled. C. speciosissimus toxin acted primarily on the epithelial cells of the proximal tubules. No changes were seen in the glomeruli. First ultrastructural changes were observed at 2 days in the renal cortex. The most prominent damage occurred at 5 days, when most of the proximal tubular cells appeared necrotic. Dark bodies (diameter 0.15-0.5 micron) spaced by 25-45 nm were frequently found in the damaged tubular cell nuclei. Regeneration of the tubular cells was seen at 10 days. After 2 months, increased amount of collagen fibres were seen between tubules. The nuclear changes in the damaged tubular cells and the slowly manifesting toxicity suggest that C. speciosissimus toxin acts on nuclei or nucleoli and/or metabolic pathways associated with them.
通过透射电子显微镜研究了剧毒蘑菇——华美丝膜菌对大鼠肾脏的影响。将干燥蘑菇悬浮液（500毫克/千克体重）作为单剂量直接经食管给药。通过灌注固定肾脏，并对皮质和髓质进行取样。华美丝膜菌毒素主要作用于近端小管的上皮细胞。肾小球未见变化。在第2天，肾皮质观察到首次超微结构变化。最显著的损伤发生在第5天，此时大多数近端小管细胞出现坏死。在受损肾小管细胞核中经常发现直径为0.15 - 0.5微米、间距为25 - 45纳米的深色小体。在第10天可见肾小管细胞再生。2个月后，小管间可见胶原纤维数量增加。受损肾小管细胞的核变化以及毒性的缓慢显现表明，华美丝膜菌毒素作用于细胞核或核仁以及/或者与之相关的代谢途径。
