有机溶剂对人红细胞膜乙酰胆碱酯酶体外活性的影响。

Effect of organic solvents on human erythrocyte membrane acetylcholinesterase activity in vitro.

作者信息

Korpela M, Tähti H

出版信息

Arch Toxicol Suppl. 1986;9:320-3. doi: 10.1007/978-3-642-71248-7_55.

Abstract

To study the effects of aromatic hydrocarbons, chlorinated aliphatic hydrocarbons, and alcohols on erythrocyte membrane, changes in the membrane-bound acetylcholinesterase (AchE) activity were tested after solvent treatment by the Ellman method. When the maximal effects of these solvents were tested at +37 degrees C, they diminished the AchE activity: benzene 75%, xylene 65%, styrene 80%, trichloroethylene 55%, tetrachloroethylene 60%, 1,1,1-trichloroethane 25%, and 1,1,2,2-tetrachloroethane 75%. Of the alcohols studied, only ethanol had a slight AchE inhibiting effect at +37 degrees C. The decrease in the incubation temperature increased the AchE inhibiting potency of aromatic hydrocarbons more than that of chlorinated aliphatic hydrocarbons and alcohols. The differences in inhibition may be due to the lipid solubility of the solvents, but also other factors, e.g. molecular structure, may determine it. Changes in the membrane-bound AchE may be one of the factors determining membrane fluidity.

摘要

为研究芳烃、氯化脂肪烃和醇类对红细胞膜的影响，采用埃尔曼法在溶剂处理后检测膜结合乙酰胆碱酯酶（AchE）活性的变化。当在37℃测试这些溶剂的最大效应时，它们降低了AchE活性：苯降低75%，二甲苯降低65%，苯乙烯降低80%，三氯乙烯降低55%，四氯乙烯降低60%，1,1,1-三氯乙烷降低25%，1,1,2,2-四氯乙烷降低75%。在所研究的醇类中，只有乙醇在37℃时有轻微的AchE抑制作用。孵育温度的降低使芳烃对AchE的抑制效力比氯化脂肪烃和醇类增加得更多。抑制作用的差异可能归因于溶剂的脂溶性，但也可能由其他因素决定，例如分子结构。膜结合AchE的变化可能是决定膜流动性的因素之一。