Department of Innovative Technologies in Medicine & Dentistry, University of Chieti-Pescara, Italy; Department of Oral Implantology, Dental Research Division, College Ingà, UNINGÁ, 29312, Cachoeiro de Itapemirim, Espirito Santo, Brazil.
Department of Innovative Technologies in Medicine & Dentistry, University of Chieti-Pescara, Italy.
J Mech Behav Biomed Mater. 2022 Feb;126:104773. doi: 10.1016/j.jmbbm.2021.104773. Epub 2021 Aug 19.
Physicochemical properties of titanium surfaces, such as wettability, influence protein binding, cell adhesion and proliferation, therefore osseointegration. The objective of this study was to investigate the wetting behaviour of two titanium surfaces, sandblasted and double acid etched (group S/E) and sandblasted (group S), using blood and Autologous Platelet Liquid (APL). Surface morphology and roughness were investigated by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The static contact angle (CA) was assessed with the sessile drop technique. The work also evaluates, with SEM observation, the fibrin clot structure that develops from blood and APL, knowing that a greater clot, firmly attached to an implant can facilitate cell migration to the implant interface. Both surfaces exhibited a hydrophobic behaviour, regardless of the wetting liquid used, but the S surface showed higher CA values for both the wetting fluids used. Lower CA values on the S/E surface are attributable to the different surface energy, which depends on different surface topography (the S surfaces were rougher) and on chemical composition. No statistically significant differences between the values of CA of blood and APL were found on the same surfaces. The clot obtained from whole blood differs from the APL clot due to a different cellular composition and fibrin density.
钛表面的理化特性，如润湿性，会影响蛋白质结合、细胞黏附和增殖，从而影响骨整合。本研究的目的是使用血液和自体血小板液（APL）来研究喷砂酸蚀（S/E）和喷砂（S）两种钛表面的润湿性。通过扫描电子显微镜（SEM）和原子力显微镜（AFM）研究表面形貌和粗糙度。采用悬滴法评估静态接触角（CA）。该研究还通过 SEM 观察评估了从血液和 APL 中形成的纤维蛋白凝块结构，因为较大的、牢固附着在植入物上的凝块可以促进细胞向植入物界面迁移。两种表面都表现出疏水性，无论使用哪种润湿液，但 S 表面对两种润湿液的 CA 值都更高。S/E 表面的 CA 值较低归因于不同的表面能，这取决于不同的表面形貌（S 表面更粗糙）和化学成分。在相同的表面上，血液和 APL 的 CA 值之间没有统计学上的显著差异。由于细胞成分和纤维蛋白密度不同，全血形成的凝块与 APL 凝块不同。
