Sun Yajun, Liu Miaomiao, Niu Mingze, Zhao Xin
College of Animal Science and Technology, Northwest A&F University, Yangling, China.
Department of Animal Science, McGill University, Montreal, QC, Canada.
Front Microbiol. 2021 Oct 7;12:709841. doi: 10.3389/fmicb.2021.709841. eCollection 2021.
Phenotypic heterogeneity within a bacterial population may confer new functionality and allow microorganisms to adapt to fluctuating environments. Previous work has suggested that could form small colony variants to avoid elimination by therapeutic antibiotics and host immunity systems. Here we show that a reversible non-pigment large colony morphology (Mu50∆-LC) was observed in Mu50 after knocking out coding for the LytR-CpsA-Psr family A protein. Mu50∆-LC increased resistance to β-lactam antibiotics, in addition, the enlarged cell size, enhanced spreading ability on solid medium, and reduced biofilm formation, suggesting better abilities for bacterial expansion. Moreover, the expression of encoding protein A was significantly increased in Mu50∆-LC. This study shows that besides the small colony variants, could fight against antibiotics and host immunity through phenotype switching into a large colony variant.
细菌群体内的表型异质性可能赋予新功能，并使微生物能够适应不断变化的环境。先前的研究表明，（此处原文缺失关键信息）可形成小菌落变体以避免被治疗性抗生素和宿主免疫系统清除。在此我们表明，在敲除编码LytR-CpsA-Psr家族A蛋白的（此处原文缺失关键信息）后，在Mu50中观察到一种可逆的非色素大菌落形态（Mu50∆-LC）。Mu50∆-LC增加了对β-内酰胺抗生素的抗性，此外，细胞尺寸增大、在固体培养基上的扩散能力增强以及生物膜形成减少，表明细菌扩张能力更强。此外，编码蛋白A的（此处原文缺失关键信息）在Mu50∆-LC中的表达显著增加。这项研究表明，除了小菌落变体之外，（此处原文缺失关键信息）可通过转变为大菌落变体的表型来对抗抗生素和宿主免疫。
