用于电子显微镜检查的血样制备：标准方案。

Preparation of blood samples for electron microscopy: The standard protocol.

作者信息

Choudhary Om Prakash, Sarkar Rupan, Chethan G E, Doley Probal Jyoti, Chandra Kalita Pranab, Kalita Arup

机构信息

Department of Veterinary Anatomy and Histology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (I), Selesih, Aizawl, 796015, Mizoram, India.

Independent Researcher, 07, Type IV Quarter, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (I), Selesih, Aizawl, 796015, Mizoram, India.

出版信息

Ann Med Surg (Lond). 2021 Oct 5;70:102895. doi: 10.1016/j.amsu.2021.102895. eCollection 2021 Oct.

DOI:10.1016/j.amsu.2021.102895

PMID:34691432

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8519801/

Abstract

Electron microscopy is a powerful tool to study biological samples at higher magnification. The higher magnifications achieved by the electron microscopes are helpful to the researchers to study surface morphology as well as cellular morphology of the samples. The blood sample surface morphology can be visualized at higher magnification by scanning electron microscope (SEM). For the examination of the blood cells at the cellular level, transmission electron microscopes (TEM) are used. In this article, we have described the step-by-step standard protocol for the preparation of blood samples for electron microscopy. The prepared blood samples can be visualized under SEM and TEM. The obtained electron micrographs of blood cells can be used for differential diagnosis of various diseases at the cellular level.

摘要

电子显微镜是一种在更高放大倍数下研究生物样本的强大工具。电子显微镜所实现的更高放大倍数有助于研究人员研究样本的表面形态以及细胞形态。通过扫描电子显微镜（SEM）可以在更高放大倍数下观察血样的表面形态。为了在细胞水平上检查血细胞，则使用透射电子显微镜（TEM）。在本文中，我们描述了用于电子显微镜检查的血样制备的分步标准方案。制备好的血样可以在SEM和TEM下进行观察。所获得的血细胞电子显微照片可用于在细胞水平上对各种疾病进行鉴别诊断。