College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, China.
ChaYeKou Town Health Center of LaiWu District, Jinan, China.
J Diabetes Res. 2021 Oct 15;2021:9949302. doi: 10.1155/2021/9949302. eCollection 2021.
The role of traditional Chinese medicine Prunella vulagaris L in the treatment of tumors and inflammation has been widely confirmed. We found that some signaling pathways of Prunella vulgaris L action can also regulate diabetes and hypertension, so we decided to study the active ingredients, potential targets and signaling pathway of Prunrlla vulgaris L, and explore the "multi-target, multi-pathway" molecular mechanism of Prunella vulgaris L on diabetes mellitus complicated with hypertension(DH). . Based on TCMSP(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform) and CNKI(China National Knowledge Infrastructure), the components and action targets related to Prunella vulgaris L were screened. The OMIM(Online Mendelian Inheritance in Man) and GeneCards (The human gene database) were used to search for targets related to DH. The "gene - drug - disease" relationship map was drawn by Cytoscape_v3.7.2 plug-in. The target was amplified by the STRING platform, and the "protein - protein" interaction relationship (PPI) network of the interacting target was obtained by the STRING online analysis platform and the Cytoscape_v3.7.2 plug-in. Finally, GO enrichment analysis and KEGG pathway enrichment analysis were conducted on David and Metascape platform to study the co-acting targets. Results. 11 active components, 41 key targets and 16 significant signaling pathways were identified from Prunella vulgaris L. The main active components of Prunella vulgaris L against DH were quercetin and kaumferol, etc, and potential action targets were IL-6 and INS, etc and signaling pathways were AGE-RAGE signaling pathway, TNF signaling pathway, MAPK signaling pathway, PI3K-AKT signaling pathway, etc. It involves in biological processes such as cell proliferation, apoptosis and inflammatory response. . The main molecular mechanism of Prunella vulgaris L against DH is that sterols and flavonoids play an active role by affecting TNF signaling pathway, AGE-RAGE signaling pathway, MAPK pathway, PI3K-Akt pathway related targets such as IL-6 and INS.
中草药夏枯草在治疗肿瘤和炎症方面的作用已得到广泛证实。我们发现，夏枯草的某些作用信号通路也可以调节糖尿病和高血压，因此我们决定研究夏枯草的活性成分、潜在靶点和信号通路，并探讨夏枯草治疗糖尿病合并高血压（DH）的“多靶点、多通路”分子机制。基于 TCMSP（中药系统药理学数据库和分析平台）和 CNKI（中国知识基础设施），筛选与夏枯草相关的成分和作用靶点。利用 OMIM（在线孟德尔遗传数据库）和 GeneCards（人类基因数据库）搜索与 DH 相关的靶点。通过 Cytoscape_v3.7.2 插件绘制“基因-药物-疾病”关系图。通过 STRING 平台对靶标进行扩增，通过 STRING 在线分析平台和 Cytoscape_v3.7.2 插件获得相互作用靶标的“蛋白质-蛋白质”相互作用网络（PPI）。最后，在 David 和 Metascape 平台上进行 GO 富集分析和 KEGG 通路富集分析，研究共同作用靶标。结果：从夏枯草中鉴定出 11 种活性成分、41 个关键靶标和 16 个显著信号通路。夏枯草治疗 DH 的主要活性成分有槲皮素、山奈酚等，潜在作用靶点有 IL-6、INS 等，信号通路有 AGE-RAGE 信号通路、TNF 信号通路、MAPK 信号通路、PI3K-Akt 信号通路等，涉及细胞增殖、凋亡、炎症反应等生物学过程。夏枯草治疗 DH 的主要分子机制是甾醇和类黄酮通过影响 TNF 信号通路、AGE-RAGE 信号通路、MAPK 通路、PI3K-Akt 通路等相关靶点如 IL-6、INS 发挥作用。
