Guo Yupeng, Yao Buqing, Da Wenting, Du Feichao, Yuan Mengran, Li Junqiao
Qinghai Provincial Key Laboratory of High Value Utilization of Characteristic Economic Plants, College of Ecological Environment and Resources, Qinghai Nationalities University, Xining, Qinghai, P. R. China.
Key Laboratory of Cold Regions Restoration Ecology, Qinghai Province, and Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, People's Republic of China.
Mitochondrial DNA B Resour. 2021 Oct 20;6(11):3250-3252. doi: 10.1080/23802359.2021.1991244. eCollection 2021.
Bunge ex Lehm. is a widespread perennial herb in north China. The plant has little yellow flowers, and the petioles are white-tomentose and sparsely villous. To determine the chloroplast genome, total genomic DNA was extracted from fresh leaves and sequenced. The complete chloroplast genome was assembled and annotated. The chloroplast genome of this plant is a circular form with a length of 156,240 bp, including a large single-copy region (LSC, 85,748 bp), a small single-copy region (SSC, 18,566 bp), and two inverted repeats (IRs, 25,963 bp). A total of 132 genes were predicted, comprising 87 encoded proteins, 8 rRNAs and 37 tRNAs. The evolutionary history indicates that was grouped within and formed a clade with and with a 100% bootstrap support value. The complete cp genome can serve as a reference for future studies on molecular biology, evolution, population genetics, taxonomy and resource protection.
Bunge ex Lehm.是中国北方一种分布广泛的多年生草本植物。该植物有小黄花，叶柄被白色绒毛且疏生柔毛。为了确定叶绿体基因组，从新鲜叶片中提取总基因组DNA并进行测序。完整的叶绿体基因组被组装和注释。该植物的叶绿体基因组呈环状，长度为156,240 bp，包括一个大单拷贝区域（LSC，85,748 bp）、一个小单拷贝区域（SSC，18,566 bp）和两个反向重复序列（IRs，25,963 bp）。共预测到132个基因，包括87个编码蛋白、8个rRNA和37个tRNA。进化历史表明，该植物被归为某一类群，并与另外两个物种形成一个分支，自展支持值为100%。完整的叶绿体基因组可为未来分子生物学、进化、群体遗传学、分类学和资源保护等研究提供参考。
