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1833年 Bunge 的完整叶绿体基因组及系统发育分析。

The complete chloroplast genome and phylogenetic analysis of Bunge 1833.

作者信息

Guo Yupeng, Yao Buqing, Yuan Mengran, Li Junqiao

机构信息

Qinghai Provincial Key Laboratory of High value Utilization of Characteristic Economic Plants, The College of Ecological Environment and Resources, Qinghai Minzu University, Xining, Qinghai, P. R. China.

Key Laboratory of Cold Regions Restoration Ecology, Qinghai Province, and Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, P. R. China.

出版信息

Mitochondrial DNA B Resour. 2021 Nov 10;6(12):3364-3366. doi: 10.1080/23802359.2021.1997108. eCollection 2021.

Abstract

Bunge 1833 is a widespread perennial herb in northern China. The plant has white flowers and white hairs on the leaves and stems. To determine the chloroplast genome, total DNA was extracted from a sample and sequenced on the Illumina HiSeq4000 platform. After sequencing, the reads of chloroplast DNA were assembled and annotated via NOVOPlasty and PGA respectively. The chloroplast genome of this plant has a circular form with a length of 123,492 bp, a 34% GC content and IR loss. After annotation, a total of 113 genes were predicted for this cp genome, comprising 79 encoded proteins, 4 rRNAs and 30 tRNAs. The evolutionary history indicates that was grouped within and formed a clade with with a 100% BS support value. The complete chloroplast genome can serve as a reference for future studies on molecular biology, evolution, population genetics, taxonomy and resource protection.

摘要

Bunge 1833是中国北方一种广泛分布的多年生草本植物。该植物开白花,叶和茎上有白毛。为了确定叶绿体基因组,从一个样本中提取了总DNA,并在Illumina HiSeq4000平台上进行测序。测序后,叶绿体DNA的读数分别通过NOVOPlasty和PGA进行组装和注释。该植物的叶绿体基因组呈环状,长度为123492 bp,GC含量为34%,存在IR缺失。注释后,该叶绿体基因组共预测出113个基因,包括79个编码蛋白、4个rRNA和30个tRNA。进化历史表明,该植物被归入某类,并与另一类形成一个分支,支持值为100%的自展值。完整的叶绿体基因组可为未来分子生物学、进化、群体遗传学、分类学和资源保护研究提供参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ceb7/8594883/50d02759ed28/TMDN_A_1997108_F0001_B.jpg

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