利用冷冻电镜观察替考拉宁超分子聚集体及细胞壁损伤情况。
Visualizing Teixobactin Supramolecular Assemblies and Cell Wall Damage in Using CryoEM.
作者信息
Hurst Paul Joshua, Morris Michael A, Graham Annissa A, Nowick James S, Patterson Joseph P
机构信息
Department of Chemistry, University of California-Irvine, Irvine, California 92697-2025, United States.
Department of Materials Science and Engineering, University of California, Irvine, California 92697, United States.
出版信息
ACS Omega. 2021 Oct 7;6(41):27412-27417. doi: 10.1021/acsomega.1c04331. eCollection 2021 Oct 19.
The antibiotic teixobactin targets bacterial cell walls. Previous research has proposed that the active form of teixobactin is a nano-/micron-sized supramolecular assembly. Here, we use cryogenic transmission electron microscopy to show that at 1 mg/mL, teixobactin forms sheet-like assemblies that selectively act upon the cell wall. At 4 μg/mL, teixobactin is active, and aggregates are formed either transiently or sparingly at the cell surface.
抗生素替考拉宁靶向细菌细胞壁。先前的研究提出，替考拉宁的活性形式是纳米/微米级的超分子聚集体。在此，我们使用低温透射电子显微镜表明，在1毫克/毫升的浓度下，替考拉宁形成片状聚集体，选择性地作用于细胞壁。在4微克/毫升的浓度下，替考拉宁具有活性，并且在细胞表面短暂或少量地形成聚集体。
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