Calderón-Chiu Carolina, Calderón-Santoyo Montserrat, Damasceno-Gomes Simone, Ragazzo-Sánchez Juan Arturo
Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México/Instituto Tecnológico de Tepic, Av. Tecnológico #2595, Col. Lagos del Country, Tepic, Nayarit C.P. 63175, México.
Center of Exact and Technological Sciences, State University of West Paraná (UNIOESTE), Cascavel, Brazil.
Food Chem X. 2021 Oct 7;12:100138. doi: 10.1016/j.fochx.2021.100138. eCollection 2021 Dec 30.
This study aimed to evaluate the encapsulating potential of a jackfruit leaf protein hydrolysate, through obtaining pentacyclic triterpenes-rich extract loaded nanoemulsion. Response surface methodology (RSM) was used to optimize the conditions to obtain an optimal nanoemulsion (NE-Opt). The effect of protein hydrolysate concentration (0.5-2%), oil loaded with extract (2.5-7.5%), and ultrasound time (5-15 min) on the polydispersity index (PDI) and droplet size of the emulsion (D[3,2] and D[4,3]) was evaluated. RSM revealed that 1.25% protein hydrolysate, 2.5% oil, and ultrasound time of 15 min produced the NE-Opt with the lowest PDI (0.85), D[3,2] (330 nm), and D[4,3] (360 nm). Encapsulation efficiency and extract loading of the NE-Opt was of 40.15 ± 1.46 and 18.03 ± 2.78% respectively. The NE-Opt was relatively stable during storage (at 4 and 25 °C), pH, temperature, and ionic strength. Then, the protein hydrolysate could be used as an alternative to conventional emulsifiers.
本研究旨在通过制备富含五环三萜提取物的纳米乳液，评估波罗蜜叶蛋白水解物的包封潜力。采用响应面法（RSM）优化条件以获得最佳纳米乳液（NE-Opt）。评估了蛋白水解物浓度（0.5-2%）、提取物负载油（2.5-7.5%）和超声时间（5-15分钟）对乳液多分散指数（PDI）和液滴尺寸（D[3,2]和D[4,3]）的影响。RSM结果表明，1.25%的蛋白水解物、2.5%的油和15分钟的超声时间可制得PDI最低（0.85）、D[3,2]为330纳米、D[4,3]为360纳米的NE-Opt。NE-Opt的包封效率和提取物负载率分别为40.15±1.46%和18.03±2.78%。NE-Opt在储存（4和25℃）、pH值、温度和离子强度方面相对稳定。因此，蛋白水解物可作为传统乳化剂的替代品。
