Laboratorio Integral de Investigacion en Alimentos, Tecnologico Nacional de Mexico-Instituto Tecnologico de Tepic, Av. Tecnologico 2595, C.P. 63175 Tepic, Nayarit, Mexico.
Departamento de Investigacion y Posgrado en Alimentos, Universidad de Sonora, C.P. 8300 Hermosillo, Sonora, Mexico.
Anticancer Agents Med Chem. 2021 Oct 28;21(16):2250-2257. doi: 10.2174/1871520621999210104201242.
Cancer is a disease characterized by the invasion and uncontrolled growth of cells. One of the best ways to minimize the harmful effects of mutagens is through the use of natural antimutagens. In this regard, the search for new antimutagens that act in the chemoprevention could represent a promising field in this area.
In this study biological potential of 11 fractions from Coccoloba uvifera L. leaf hexane extract was evaluated by several in vitro tests.
Leaves were lyophilized and hexane extraction was performed. The extract was fractionated by column chromatography with hexane, ethyl acetate, and methanol. The antimutagenic (Ames test), antiproliferative (MTT test), and antioxidant capacity (DPPH, ABTS, and ferrous ion chelation) of the fractions were evaluated.
Fractions 4, 6, 8, and 9 have antimutagenic activity (against sodium azide in strain TA100), fraction 11 showed antiproliferative capacity (IC of 24 ± 9 μg/mL in cells of HCT 116). The fractions with the highest activity were analyzed by HPLC-MS and lupeol, acacetin, and β-sitosterol were identified.
This study demonstrates, for the first time, the bioactivity of C. uvifera leaf as a new source of High Biological Value Compounds (HBVC), which can be of interest to the food and pharmaceutical industries.
癌症是一种以细胞侵袭和不受控制生长为特征的疾病。最大限度地减少诱变剂有害影响的最佳方法之一是使用天然抗诱变剂。在这方面，寻找作用于化学预防的新抗诱变剂可能代表了该领域的一个有前途的领域。
本研究通过多项体外试验评估了 Coccoloba uvifera L. 叶己烷提取物的 11 个馏分的生物学潜力。
将叶片冻干并用己烷进行提取。用己烷、乙酸乙酯和甲醇通过柱层析对提取物进行分级。评估馏分的抗诱变（Ames 试验）、抗增殖（MTT 试验）和抗氧化能力（DPPH、ABTS 和亚铁离子螯合）。
馏分 4、6、8 和 9 具有抗诱变活性（对 TA100 菌株中的叠氮化钠），馏分 11 显示出抗增殖能力（在 HCT 116 细胞中的 IC 为 24 ± 9 μg/mL）。对活性最高的馏分进行了 HPLC-MS 分析，鉴定出羽扇豆醇、乙酰丁香酮和β-谷甾醇。
本研究首次证明了 Coccoloba uvifera 叶作为高生物价值化合物（HBVC）的新来源的生物活性，这可能对食品和制药行业具有重要意义。
