两种人皮肤血管肉瘤细胞系KU-CAS3和KU-CAS5的建立与鉴定

Establishment and characterization of two human cutaneous angiosarcoma cell lines, KU-CAS3 and KU-CAS5.

作者信息

You Hi-Jin, Kim Hyung-Kyu, Kim Min-Sook, Lee Yun-Hwan, Chung Jae-Ho, Hwang Na-Hyun, Kim Deok-Woo, Choi Jung-Woo, Lee Ju-Han

机构信息

Department of Plastic and Reconstructive Surgery, Korea University Medicine, Ansan, South Korea.

Department of Pathology, Korea University Medicine, Ansan, South Korea.

出版信息

Head Neck. 2022 Jan;44(1):7-17. doi: 10.1002/hed.26886. Epub 2021 Oct 24.

DOI:10.1002/hed.26886

PMID:34693591

Abstract

BACKGROUND

Cutaneous angiosarcoma (CAS) is a rare but fatal cancer. Established CAS cell lines are necessary for the investigation of their properties and treatment options.

METHODS

Two cell lines, KU-CAS3 and KU-CAS5, were established from human angiosarcoma specimens obtained from the scalp. Flow cytometric assay, tube formation assay, low-density lipoprotein (LDL) uptake assay, immunofluorescence analysis, real-time PCR, tumorigenesis assay, and STR analysis were conducted.

RESULTS

The cells showed endothelial cell properties, based on the cobblestone appearance upon reaching confluence, CD31 positivity, tube-formation activity, active uptake of acetylated LDL, and vWF expression. The two cell lines expressed relatively high levels of adrenergic β2 receptor, and the VEGF1 and VEGF2 receptors. In the in vivo study, the growing neoplasms, confirmed as CAS, were identified as subcutaneous dark papules. KU-CAS cell lines were considered authentic based on STR profiling.

CONCLUSIONS

KU-CAS3 and KU-CAS5 are the first human CAS cell lines having tumorigenic potential in vivo.

摘要

背景

皮肤血管肉瘤（CAS）是一种罕见但致命的癌症。建立CAS细胞系对于研究其特性和治疗方案是必要的。

方法

从头皮获取的人类血管肉瘤标本中建立了两个细胞系，KU-CAS3和KU-CAS5。进行了流式细胞术分析、管形成分析、低密度脂蛋白（LDL）摄取分析、免疫荧光分析、实时PCR、肿瘤发生分析和STR分析。

结果

这些细胞表现出内皮细胞特性，汇合时呈鹅卵石样外观、CD31阳性、管形成活性、乙酰化LDL的活跃摄取以及vWF表达。这两个细胞系表达相对高水平的肾上腺素能β2受体以及VEGF1和VEGF2受体。在体内研究中，生长的肿瘤被确认为CAS，表现为皮下深色丘疹。基于STR分析，KU-CAS细胞系被认为是真实的。