Department of Medical Oncology, Radboud University Medical Center, Geert Grooteplein Zuid 8, 6525 GA, Nijmegen, The Netherlands.
Central Animal Facility, Radboud University Medical Center, Nijmegen, The Netherlands.
Sci Rep. 2023 Feb 14;13(1):2653. doi: 10.1038/s41598-023-29569-x.
Angiosarcomas are a heterogeneous group of rare endothelial malignancies with a complex, not completely unravelled biology. They encompass primary (sporadically occurring) angiosarcomas of several origins and secondary angiosarcomas, which often arise due to DNA damaging factors including radiotherapy or ultraviolet light exposure. The optimal treatment of metastatic angiosarcomas is unclear and the prognosis is poor. In order to discover novel treatment strategies for angiosarcomas it is important to take the heterogeneity of these tumors into account. For this reason it is also important to have preclinical models available for the different clinical subtypes. Owing to the rarity of angiosarcomas, models are scarce. So far, only five human cell lines of angiosarcomas (all of the scalp after UV exposure) are available worldwide. In this paper we describe a novel established patient-derived xenograft model of a radiotherapy-induced angiosarcoma of the breast. The tumor was characterized by a MYC amplification, CD31 and ERG immunohistochemical positivity and was further characterized by using next generation sequencing (TruSight Oncology 500) in combination with the R-package XenofilteR to separate mouse from human sequence reads.
血管肉瘤是一组罕见的内皮恶性肿瘤，具有复杂的、尚未完全阐明的生物学特性。它们包括多种起源的原发性（偶发性）血管肉瘤和继发性血管肉瘤，后者通常由于包括放射治疗或紫外线暴露在内的 DNA 损伤因素而发生。转移性血管肉瘤的最佳治疗方法尚不清楚，预后较差。为了发现血管肉瘤的新治疗策略，考虑到这些肿瘤的异质性非常重要。出于这个原因，拥有不同临床亚型的临床前模型也很重要。由于血管肉瘤罕见，模型稀缺。到目前为止，全世界只有五种血管肉瘤的人类细胞系（均来自紫外线照射后的头皮）。在本文中，我们描述了一种新建立的放射诱导性乳腺血管肉瘤患者来源异种移植模型。该肿瘤表现为 MYC 扩增、CD31 和 ERG 免疫组织化学阳性，并进一步通过使用下一代测序（TruSight Oncology 500）结合 R 包 XenofilteR 来分离小鼠和人序列读数进行了特征描述。
