营养状况显著影响疾控中心瓶生物测定中的毒理学终点。

Nutritional status significantly affects toxicological endpoints in the CDC bottle bioassay.

机构信息

Emerging Pathogens Institute, Department of Entomology and Nematology, University of Florida, Gainesville, FL, USA.

出版信息

Pest Manag Sci. 2022 Feb;78(2):743-748. doi: 10.1002/ps.6687. Epub 2021 Nov 17.

DOI:10.1002/ps.6687

PMID:34693614

Abstract

BACKGROUND

The CDC Bottle Bioassay serves as an inexpensive and effective way to screen field-caught mosquitoes against a wide variety of insecticidal active ingredients and commercial formulations, with the goal of detecting resistant individuals. For this study, we assessed how sucrose-water (10% w/v) feeding status impacted the response of Aedes aegypti mosquitoes to select insecticides.

RESULTS

Starvation for 24 or 48 h decreased permethrin and malathion mean survival time by about 40%, with little difference in the two starvation times. Similar findings were also observed in a pyrethroid-resistant Puerto Rico strain challenged with permethrin, but these effects were less pronounced. To test the impact of mosquito weight, we measured weight under different 48-h nutritional conditions and found that sugar-water-fed and sugar-only-fed individuals were approximately the same weight (ANOVA, Bonferroni post-test P value >0.999) and that individuals fed water only were considerably lighter than either sugar-water-fed (ANOVA, Bonferroni post-test P value = 0.034) or sugar-only-fed individuals (ANOVA, Bonferroni post-test P value = 0.027) but equal in weight to starved mosquitoes (ANOVA, Bonferroni post-test P value >0.99). Of the nutritional challenges, water-only-fed mosquitoes were the most insecticide tolerant (ANOVA, Bonferroni post-test P values to all other treatments <0.01).

CONCLUSIONS

The results indicate insect nutritional status is an important experimental variable, particularly the hydration status of mosquitoes shortly before insecticide exposure. Moreover, as significant differences were observed between starved and component-fed (water, sugar, or sugar and water) mosquitoes in a pyrethroid-resistant strain, some caution is appropriate when interpreting resistance/susceptibility diagnoses with this bioassay. © 2021 Society of Chemical Industry. This article has been contributed to by US Government employees and their work is in the public domain in the USA.

摘要

背景

疾病预防控制中心瓶生物测定法是一种廉价且有效的方法，可用于筛选野外捕获的蚊子对各种杀虫活性成分和商业制剂的抗性，目的是检测抗性个体。在这项研究中，我们评估了蔗糖水（10%w/v）喂养状态如何影响埃及伊蚊对选择杀虫剂的反应。

结果

饥饿 24 或 48 小时会使氯菊酯和马拉硫磷的平均存活时间缩短约 40%，而这两种饥饿时间的差异很小。在接受氯菊酯挑战的抗药性波多黎各品系中也观察到了类似的发现，但这些影响不太明显。为了测试蚊子体重的影响，我们测量了在不同 48 小时营养条件下的体重，发现糖-水喂养和仅糖喂养的个体体重大致相同（方差分析，Bonferroni 后测 P 值>0.999），而仅水喂养的个体比糖-水喂养的个体（方差分析，Bonferroni 后测 P 值=0.034）或仅糖喂养的个体（方差分析，Bonferroni 后测 P 值=0.027）轻得多，但与饥饿的蚊子体重相等（方差分析，Bonferroni 后测 P 值>0.99）。在这些营养挑战中，仅水喂养的蚊子对杀虫剂的耐受性最强（方差分析，Bonferroni 后测 P 值对所有其他处理<0.01）。