Dengue Branch, Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, San Juan, Puerto Rico.
Department of Entomology, University of California, Riverside, CA, USA.
J Med Entomol. 2022 Jan 12;59(1):314-319. doi: 10.1093/jme/tjab162.
Mass-trapping has been used to control outbreaks of Aedes aegypti (Linnaeus) (Diptera: Culicidae) in Puerto Rico since 2011. We investigated the effect of multi-year, insecticide-free mass trapping had on the insecticide susceptibility profile of Ae. aegypti. Eggs collected in southern Puerto Rico were used to generate F1 populations that were tested for susceptibility to permethrin, sumethrin, bifenthrin, deltamethrin, and malathion according to CDC bottle bioassays protocols. All populations of Ae. aegypti were resistant to the synthetic pyrethroids and mosquitoes from two locations were partially resistant to malathion. Population genetic analysis, using a double digest restriction sites associated DNA sequencing (ddRADseq) approach, indicated a large amount of migration between study sites effectively homogenizing the mosquito populations. Mass-trapping using noninsecticidal autocidal gravid ovitraps did not restore susceptibility to five active ingredients that are found in commercial insecticides. Migration between communities was high and would have brought outside alleles, including resistant alleles to the treatment communities. Further investigation suggests that household use of commercially available insecticide products may continue to select for resistance in absence of public health space spraying of insecticides.
自 2011 年以来，大规模诱捕已被用于控制波多黎各的埃及伊蚊（Linnaeus）（双翅目：蚊科）疫情。我们研究了多年无杀虫剂大规模诱捕对埃及伊蚊杀虫剂敏感性的影响。在波多黎各南部收集的卵用于产生 F1 种群，根据疾病预防控制中心瓶生物测定协议测试它们对氯菊酯、丙硫磷、溴氰菊酯、溴氰菊酯和马拉硫磷的敏感性。所有埃及伊蚊种群对合成拟除虫菊酯均有抗性，来自两个地点的蚊子对马拉硫磷有部分抗性。使用双酶切相关 DNA 测序（ddRADseq）方法进行种群遗传分析表明，研究地点之间存在大量的迁移，有效地使蚊子种群同质化。使用非杀虫性自杀死卵诱捕器进行的大规模诱捕并没有恢复对五种存在于商业杀虫剂中的活性成分的敏感性。社区之间的迁移率很高，会将外来等位基因，包括抗性等位基因，带入处理社区。进一步的研究表明，在没有公共卫生空间喷洒杀虫剂的情况下，家用市售杀虫剂产品的使用可能会继续选择产生抗药性。
