Department of Animal Production (Animal Physiology), Faculty of Agriculture, Fayoum University, Fayoum, Egypt.
Botany Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
Int J Radiat Biol. 2022;98(2):191-204. doi: 10.1080/09553002.2022.1998702. Epub 2021 Nov 8.
Oxidative stress and reactive oxygen species (ROS) are primarily responsible for the development of male infertility after exposure to γ-irradiation. The present work aimed to assess the ameliorative and therapeutic roles of the aqueous and ethanolic extracts of the edible seaweed () on spermatogenesis and infertility in γ-irradiated Wistar rats.
Induction of infertility was performed by exposing the rats to Cs-gamma rays, using a single dose of 3.5 Gy. γ-irradiated rats were given the ethanolic (-EtOH) and aqueous extracts intraperitoneally on a daily base for two consecutive weeks at doses of 100 and 400 mg/kg body weight (b.wt.) for each seaweed extract. Morphometric data of the testes, semen quality indices, antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and glutathione peroxidase (GPx), and deoxyribonucleic acid (DNA) fragmentation were assessed. The results obtained were taken during two-time intervals of 15 and 60 days from the commencement of the algal treatments. In vitro antioxidant assays and polyphenolic compounds of were characterized.
Significant negative changes in the semen quality and morphometric data of the testes, as well as remarkable DNA fragmentation, were detected in the irradiated rats compared to the control. The levels of the endogenous antioxidant enzymes (SOD, CAT, GSH, and GPx) were also significantly diminished. Nonetheless, treatments of γ-irradiated rats with the -EtOH and aqueous extracts significantly improved the above-mentioned enzymes, in addition to noteworthy amendments in the dimensions of the testes, the semen quality, as well as the DNA structure.
The ameliorative potency of to cure γ-irradiation-induced male infertility, particularly 400 mg/kg ethanolic extract for 60 days, is the result of the consistent therapeutic interventions of its potent antioxidant and anti-apoptotic polyphenols, particularly protocatechuic, -hydroxybenzoic, rosmarinic, chlorogenic, cinnamic and gentisic acids, as well as the flavonoids catechin, hesperidin, rutin and quercetin. Besides its high-value nutraceutical importance, could be a natural candidate for developing well-accepted radioprotectant products capable of treating γ-irradiation-induced male infertility.
氧化应激和活性氧（ROS）是 γ 射线照射后导致男性不育的主要原因。本研究旨在评估可食用海藻（）的水提物和醇提物对 γ 射线照射 Wistar 大鼠生殖和不育的改善和治疗作用。
通过单次给予大鼠 3.5Gy 的 Cs-γ射线诱导不育。γ 射线照射大鼠每天腹腔内给予两种海藻提取物，即 100 和 400mg/kg 体重的醇提物（-EtOH）和水提物，连续两周。评估睾丸的形态计量学数据、精液质量指数、抗氧化酶超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GPx）以及脱氧核糖核酸（DNA）碎片化。从海藻处理开始的 15 和 60 天两个时间段内获得结果。对进行体外抗氧化测定和多酚化合物分析。
与对照组相比，照射大鼠的精液质量和睾丸形态计量学数据显著恶化，以及显著的 DNA 碎片化。内源性抗氧化酶（SOD、CAT、GSH 和 GPx）水平也显著降低。然而，用 -EtOH 和水提物治疗 γ 射线照射大鼠显著改善了上述酶，同时睾丸尺寸、精液质量以及 DNA 结构也有显著改善。
（）对治疗 γ 射线照射引起的男性不育的改善作用，特别是 400mg/kg 醇提物治疗 60 天，是其有效抗氧化和抗凋亡多酚，特别是原儿茶酸、-羟基苯甲酸、迷迭香酸、绿原酸、肉桂酸和没食子酸以及类黄酮儿茶素、橙皮苷、芦丁和槲皮素的持续治疗干预的结果。除了其高营养价值外，（）还可能是开发可接受的放射性保护产品的天然候选物，这些产品能够治疗 γ 射线照射引起的男性不育。
