Neuroscience and Mental Health Institute, University of Alberta, Edmonton, Canada.
Department of Psychology, University of Alberta, Edmonton, Canada.
PLoS One. 2021 Oct 25;16(10):e0258939. doi: 10.1371/journal.pone.0258939. eCollection 2021.
Urethane, an acute laboratory anesthetic, produces distinct neurophysiological and physiological effects creating an effective model of the dynamics of natural sleep. As a model of both sleep-like neurophysiological activity and the downstream peripheral function urethane is used to model a variety of physiological and pathophysiological processes. As urethane is typically administered as a single-bolus dose, it is unclear the stability of peripheral physiological functions both within and between brain-states under urethane anesthesia. In this present study, we recorded respiration rate and heart rate concurrently with local field potentials from the neocortex and hippocampus to determine the stability of peripheral physiological functions within and between brain-states under urethane anesthesia. Our data shows electroencephalographic characteristics and breathing rate are remarkable stable over long-term recordings within minor reductions in heart rate on the same time scale. Our findings indicate that the use of urethane to model peripheral physiological functions associated with changing brain states are stable during long duration experiments.
氨基甲酸乙酯，一种急性实验室麻醉剂，会产生明显的神经生理和生理效应，为自然睡眠动态创建一个有效的模型。作为类似睡眠的神经生理活动和下游外周功能的模型，氨基甲酸乙酯被用于模拟各种生理和病理生理过程。由于氨基甲酸乙酯通常作为单次推注剂量给药，因此尚不清楚在氨基甲酸乙酯麻醉下，脑状态内和脑状态之间的外周生理功能的稳定性。在本研究中，我们记录了来自大脑皮层和海马体的局部场电位的同时的呼吸率和心率，以确定在氨基甲酸乙酯麻醉下脑状态内和脑状态之间的外周生理功能的稳定性。我们的数据表明，在长时间记录期间，脑电图特征和呼吸率非常稳定，而在同一时间尺度上，心率略有降低。我们的发现表明，在长时间实验期间，使用氨基甲酸乙酯来模拟与脑状态变化相关的外周生理功能是稳定的。
