Yew N S, Choi H R, Gallarda J L, Engel J D
Proc Natl Acad Sci U S A. 1987 Feb;84(4):1035-9. doi: 10.1073/pnas.84.4.1035.
We have isolated a cDNA clone encoding part of protein 4.1, an integral component of the erythrocyte cytoskeleton. The recombinant was isolated by immunological screening of a chicken erythroid lambda gt11 cDNA library using a monoclonal antibody directed against protein 4.1. DNA blot analysis shows that the gene is present as a single copy per haploid chicken genome, while RNA blot analysis reveals the presence of a single mRNA of 7 kilobases in reticulocytes. Message of the same size (in reduced amounts) is also present in an erythroleukemic cell line transformed by avian erythroblastosis virus and is also present in vastly reduced quantities in nonerythroid hemopoietic cells. Immunoblotting and immunofluorescence experiments show that a subset of the chicken 4.1 variant proteins is preferentially expressed during in vitro differentiation of chicken erythroleukemic cells. These data indicate that the gene is both actively transcribed and translated during early erythroid cellular maturation.
我们分离出了一个编码红细胞细胞骨架的组成成分蛋白4.1部分序列的cDNA克隆。该重组体是通过用针对蛋白4.1的单克隆抗体对鸡红细胞λgt11 cDNA文库进行免疫筛选而分离得到的。DNA印迹分析表明，该基因在单倍体鸡基因组中以单拷贝形式存在，而RNA印迹分析显示，网织红细胞中存在一种7千碱基的单一mRNA。同样大小的信使RNA（数量减少）也存在于由禽成红细胞增多症病毒转化的红白血病细胞系中，并且在非红细胞造血细胞中的含量也大幅减少。免疫印迹和免疫荧光实验表明，鸡4.1变体蛋白的一个亚群在鸡红白血病细胞的体外分化过程中优先表达。这些数据表明，该基因在早期红细胞细胞成熟过程中既被积极转录又被翻译。
