猪中丝聚合蛋白-2的表达水平与滴水损失的关系。
Expression levels of filaggrin-2 in relation to drip loss in pigs.
作者信息
Kayan Autchara, Koomkrong Nunyarat
机构信息
Department of Animal Science, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
Department of Animal Science, Faculty of Science and Technology, Suratthani Rajabhat University, Suratthani 84100, Thailand.
出版信息
Anim Biosci. 2022 Apr;35(4):624-630. doi: 10.5713/ab.21.0220. Epub 2021 Oct 21.
OBJECTIVE
The aim of this study was to investigate the expression level of filaggrin-2 (FLG2) in correlation with drip loss.
METHODS
The muscle samples were randomly taken from a local meat supplier. Samples were taken from Longissimus lumborum muscles to evaluate the drip loss (n = 100). Five muscles per group (low and high drip loss) were selected to evaluate FLG2 mRNA and protein expression levels.
RESULTS
mRNA of FLG2 gene was not significantly different in pigs with different levels of drip loss (p>0.05). Statistical analysis revealed that FLG2 protein expression levels were significantly different between the drip loss groups. Western blot revealed that the high drip loss group had higher FLG2 protein expression level than the low drip loss group (p<0.001). Moreover, immunohistochemistry revealed the high signal intensity was on the muscle cell membrane and cytoplasm.
CONCLUSION
FLG2 protein might play roles in drip loss of pork and will provide the basis for information to improving meat quality traits in pigs.
目的
本研究旨在探究丝聚合蛋白-2（FLG2）的表达水平与滴水损失之间的相关性。
方法
肌肉样本随机取自当地一家肉类供应商。从腰大肌中取样以评估滴水损失（n = 100）。每组（低滴水损失和高滴水损失）选取五块肌肉来评估FLG2 mRNA和蛋白质表达水平。
结果
不同滴水损失水平的猪中，FLG2基因的mRNA无显著差异（p>0.05）。统计分析表明，滴水损失组之间的FLG2蛋白质表达水平存在显著差异。蛋白质免疫印迹法显示，高滴水损失组的FLG2蛋白质表达水平高于低滴水损失组（p<0.001）。此外，免疫组织化学显示高信号强度位于肌细胞膜和细胞质上。
结论
FLG2蛋白可能在猪肉滴水损失中发挥作用，并将为改善猪的肉质性状提供信息依据。
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