Kaewkot Aungsuma, Boonkaewwan Chaiwat, Noosud Jatuporn, Kayan Autchara
Department of Animal Science, Kasetsart University, Bangkok, Thailand.
Department of Companion Animals Clinical Sciences, Kasetsart University, Bangkok, Thailand.
Anim Sci J. 2017 Nov;88(11):1855-1859. doi: 10.1111/asj.12863. Epub 2017 Jul 5.
Drip loss is an important meat quality trait of fresh meat affecting economic losses. The cytochrome P450c21 (CYP21) protein has a role on cortisol production and depends on stress. This might affect meat quality. The present study aimed to investigate the expression of CYP21 protein in correlation with drip loss. The samples were taken from the Longissimus dorsi muscle to evaluate drip loss (n = 300). Five muscles per group (low and high drip loss) were selected to evaluate CYP21 protein expression levels. Statistical analysis revealed that CYP21 protein expression levels were significantly difference between the drip loss groups. The high drip loss group had higher CYP21 protein expression levels than the low drip loss group (P < 0.05). Moreover, the high drip loss group had higher optical density values of the CYP21 protein band than the low drip loss group (P < 0.05). In conclusion, the expression of CYP21 protein will provide the basis for information and better understanding of the mechanisms related to drip loss in pork. Further study is warranted to validate these results in other populations.
滴水损失是影响经济损失的鲜肉重要肉质性状。细胞色素P450c21（CYP21）蛋白在皮质醇生成中起作用，且依赖于应激。这可能会影响肉质。本研究旨在探究CYP21蛋白表达与滴水损失的相关性。从背最长肌采集样本以评估滴水损失（n = 300）。每组（低滴水损失和高滴水损失）选取五块肌肉来评估CYP21蛋白表达水平。统计分析显示，滴水损失组之间CYP21蛋白表达水平存在显著差异。高滴水损失组的CYP21蛋白表达水平高于低滴水损失组（P < 0.05）。此外，高滴水损失组CYP21蛋白条带的光密度值高于低滴水损失组（P < 0.05）。总之，CYP21蛋白的表达将为了解猪肉滴水损失相关机制提供信息基础。有必要进一步研究以在其他群体中验证这些结果。
