饲养策略和老化时间会改变布拉福德阉牛的钙蛋白酶系统蛋白活性和肉质。
Feeding strategies and ageing time alter calpain system proteins activities and meat quality of Braford steers.
作者信息
Coria María Sumampa, Pighin Dario, Grigioni Gabriela, Palma Gustavo Adolfo
机构信息
Animal Production and Reproduction Laboratory, NOA Institute of Bionanotechnology (INBIONATEC), Villa El Zanjón, Santiago del Estero, G4206XCP, Argentina.
Institute for the Agricultural Development of the Semiarid (INDEAS), Faculty of Agronomy and Agroindustry (FAyA), National University of Santiago del Estero (UNSE), Santiago del Estero, G4200ABT, Argentina.
出版信息
Anim Biosci. 2022 Feb;35(2):272-280. doi: 10.5713/ab.21.0227. Epub 2021 Oct 21.
OBJECTIVE
The aim of this study was to evaluate the effect of ageing and feeding strategies on the calpain protease system and meat quality traits in Braford steers.
METHODS
Thirty Braford steers were employed; 15 animals were supplemented with corn silage during finishing and 15 were kept only on pasture. Meat quality traits and calpain system protein activity were evaluated in longissimus thoracis et lumborum (LTL) steaks aged for 2, 7, 14, and 21 days.
RESULTS
Aged meat showed higher pH and calcium content, while Warner Bratzler shear force (WBSF) decreased to day 21. No interaction between ageing and diet was seen for quality traits. Steers finished with corn silage showed higher values of water holding capacity, WBSF and free calcium, and lower values of pH and cooking loss. Calpain and calpastatin activities decreased with ageing. Finishing steers on pasture produced higher values of calpains and lower values of calpastatin activities. The higher values of calpain 1 activity were observed in muscles aged 2 days from pasture finished animals, and the lower activity of the inhibitor in the 21 days aged samples of the same group.
CONCLUSION
These results suggest a diet by ageing interaction in calpains and calpastatin and this interaction impact in Warner Bratzler Shear Force in Braford LTL muscle.
目的
本研究旨在评估衰老和饲养策略对布拉福德阉牛钙蛋白酶系统及肉质性状的影响。
方法
选用30头布拉福德阉牛；15头在育肥期补饲玉米青贮料，15头仅以牧场草料为食。对长背腰最长肌（LTL）牛排进行2、7、14和21天的成熟处理，评估其肉质性状和钙蛋白酶系统蛋白活性。
结果
成熟肉的pH值和钙含量更高，而华纳·布拉茨勒剪切力（WBSF）在第21天时下降。肉质性状方面未观察到成熟和日粮之间的相互作用。补饲玉米青贮料的阉牛的持水力、WBSF和游离钙值更高，pH值和蒸煮损失值更低。钙蛋白酶和钙蛋白酶抑制蛋白的活性随衰老而降低。以牧场草料育肥的阉牛的钙蛋白酶活性较高，钙蛋白酶抑制蛋白的活性较低。在以牧场草料育肥的动物2天龄肌肉中观察到钙蛋白酶1活性较高，而在同一组21天龄样本中抑制剂活性较低。
结论
这些结果表明钙蛋白酶和钙蛋白酶抑制蛋白存在日粮与成熟的相互作用，且这种相互作用影响布拉福德LTL肌肉的华纳·布拉茨勒剪切力。
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